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Stranica 1 iz 17 rezultatima
Adenine phosphoribosyltransferase in plant tissues: some effects of kinetin on enzymic activity.
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Adenine phosphoribosyltransferase activity was measured in extracts of soybean (Glycine max var. Acme) callus and of senescing barley leaves (Hordeum distichon c.v. Prior). The enzyme from soybean callus had Michaelis constants for adenine and 5-phosphoribosyl pyrophosphate of 1.5 and 7.5 mum
Kinetin-induced stimulation of electrogenic pumping in soybean suspension cultures is unrelated to signal transduction.
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Primary modes of action of cytokinins have been thought to involve stimulation of the electrogenic H(+) pump and-or opening of plasmamembrane Ca(2+) channels. In order to test these hypotheses, rapid changes in membrane transport in response to cytokinin application were studied in heterotrophic
Somatic embryogenesis and plantlet regeneration in the soybean Glycine max.
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A tissue culture procedure for the regeneration of somatic embryos and plantlets from somatic cells of the soybean Glycine max is described. Bean pods of soybean cv. TGM119 were immersed in liquid nitrogen for 20 minutes. Young embryos were excised from the immature seeds and cultured to form calli.
Hormonal control of cell proliferation and xylem differentiation in cultured tissues of Glycine max var. Biloxi.
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The relationship between tracheary element differentiation, cell proliferation and growth hormones was examined in agar-grown soybean callus. The time course of cell division and tracheary element formation in tissues grown on a medium containing 5 x 10(-7)m kinetin and 10(-5)m NAA was determined by
Cytokinin modification of metabolism of p-coumaric acid by a cell suspension of soybean (Glycine max (L.) Merrill).
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Cells of a soybean tissue strain suspended in an aerated liquid medium caused the disappearance of p-coumaric acid from the medium. The rate of disappearance was modified by cytokinins. When the coumarate and the cytokinin were added to the medium simultaneously, disappearance was increased if the
Cytokinin inhibition of respiration by cells and mitochondria of soybean, Glycine max (L.) Merrill.
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Cells of a soybean tissue strain, suspended in an aerated liquid medium, caused disappearance of p-coumaric acid from the medium and oxidation of guaiacol, benzidine, pyrogallol, L-dihydroxyphenylalanine and L-epinephrine. Both the disappearance and the oxidations were inhibited by
Exo-ethylene application mitigates waterlogging stress in soybean (Glycine max L.).
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BACKGROUND
Waterlogging (WL) is a key factor hindering soybean crop productivity worldwide. Plants utilize various hormones to avoid various stress conditions, including WL stress; however, the physiological mechanisms are still not fully understood.
RESULTS
To identify physiological mechanisms
Substituted nitroguanidines provide cytokinin activity during in vitro cultivation of plant tissues.
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Synthetic nitroguanidine derivatives can be used as alternatives to the traditional adenine-containing cytokinins used in plant tissue culture. First, nitroguanidine derivatives (NG) mimicked the typical activity of two standard cytokinins, 6-benzylaminopurine (BAP) and 2-isopentenyladenine (2iP) in
Gravitropism in higher plant shoots. IV. Further studies on participation of ethylene.
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Ethylene at 1.0 and 10.0 cubic centimeters per cubic meter decreased the rate of gravitropic bending in stems of cocklebur (Xanthium strumarium L.) and tomato (Lycopersicon esculentum Mill), but 0.1 cubic centimeter per cubic meter ethylene had little effect. Treating cocklebur plants with 1.0
First Report of Soybean Rust Caused by Phakopsora pachyrhizi in Ghana.
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Nigeria is the only country in West Africa where soybean rust, caused by Phakopsora pachyrhizi, has been officially reported (1). During a disease survey in Ghana during October 2006, soybean (Glycine max) leaves with rust symptoms (tan, angular lesions with erumpent sori exuding urediniospores)
Fusarium tricinctum T-2 Toxin Inhibits Auxin-promoted Elongation in Soybean Hypocotyl.
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T-2 toxin, a mycotoxin produced by Fusarium tricinctum, inhibited elongation of excised hypocotyl sections of Glycine max var. Hawkeye 63. Auxin-promoted elongation was inhibited more severely than was control elongation, and a 1 hour preincubation of 5 mum toxin prevented the induction of a faster
Changes in the pattern of phospholipid synthesis during the induction by cytokinin of cell division in soybean suspension cultures.
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A method is described for preparing fully viable, cytokinin-starved soybean (Glycine max (L.) Merr. cv. Acme) cells from a suspension-culture of callus tissue. The cells respond to kinetin treatment by re-initiating cell division. We present evidence, from the pattern of incorporation of
Effects of cytokinins on the respiration of soybean callus tissue.
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A technique which incorporates a brief blending step to disperse callus tissue into small clumps of cells was developed, and the effects of cytokinins on respiration of soybean (Glycine max [L.] Merrill var. Acme) callus tissue prepared in this way were studied. Adenine alone did not affect
Efficient soybean regeneration and Agrobacterium-mediated transformation using a whole cotyledonary node as an explant.
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An optimized regeneration and Agrobacterium-mediated transformation protocol based on whole cotyledonary node explants was developed in soybean (Glycine max) cultivar Zhong Huang 13. Adding 6-benzylaminopurine (BAP) in a germinating medium could significantly increase regeneration efficiency; the
Adenine binding sites of the lectin from lima beans (Phaseolus lunatus).
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A single high-affinity binding site for adenine and related compounds was identified in the lima bean lectin (LBL) component III tetramer. This site is identical with the high affinity site for 2,6-toludinyl-naphthalenesulfonate described previously (Roberts, D. D., and Goldstein, I. J. (1982) J.
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