phospholipase d/soja

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Phospholipase D from soybean (Glycine max L.) suspension-cultured cells: purification, structural and enzymatic properties.

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Phospholipase D (phosphatidylcholine phosphatidohydrolase EC 3.1.4.4) from soybean (Glycine max L.) suspension-cultured cell was purified around 1,200-fold to homogeneity by acetone precipitation, Macro-Prep High Q anion exchange, and octyl-Sepharose CL-4B affinity chromatography. The purified

Genomic analysis of phospholipase D family and characterization of GmPLDαs in soybean (Glycine max).

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Phospholipase D (PLD) and its product phosphatidic acid play important roles in the regulation of plant growth, development, and stress responses. The genome database analysis has revealed PLD family in Arabidopsis, rice, poplar and grape. In this study, we report a genomic analysis of 18 putative

Timecourse microarray analyses reveal global changes in gene expression of susceptible Glycine max (soybean) roots during infection by Heterodera glycines (soybean cyst nematode).

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Changes in gene expression within roots of Glycine max (soybean), cv. Kent, susceptible to infection by Heterodera glycines (the soybean cyst nematode [SCN]), at 6, 12, and 24 h, and 2, 4, 6, and 8 days post-inoculation were monitored using microarrays containing more than 6,000 cDNA inserts.

Transphosphatidylation by immobilized phospholipase D in aqueous media.

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Phospholipase D (PLD) from Streptomyces sp. was immobilized by covalent binding to aminopropyl-glass activated by glutardialdehyde and to the macroporous synthetic polymer VA-Epoxy Biosynth (from Riedel-de Häen, Seelze, Germany) pre-activated by epoxy groups. The immobilized PLDs were examined for

Phosphatidic acid activates a wound-activated MAPK in Glycine max.

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Many plant species demonstrate a systemic increase in phosphatidic acid (PA) levels after being wounded (Lee et al., 1997). To understand the role of PA in wound signal transduction, we investigated if PA can activate protein kinases in soybean (Glycine max L.). We found that a MAPK is activated in

Phospholipid composition of a plasma membrane-enriched fraction from developing soybean roots.

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Phospholipid polar head group and fatty acid composition were determined for plasma membrane enriched fractions from developing soybean root (Glycine max [L.] Merr. cult. Wells II). Plasma membrane vesicles were isolated from meristematic and mature sections of four-day-old dark grown soybean roots

Overexpression of soybean GmPLDγ enhances seed oil content and modulates fatty acid composition in transgenic Arabidopsis.

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Phospholipase D (PLD) hydrolyzes the phosphodiester bond of glycerophospholipids to yield phosphatidic acid (PA) and a free headgroup. PLDs are important for plant growth, development, and responses to external stresses. However, their roles in triacylglycerol (TAG) synthesis are still unclear.

Incorporation of choline and ethanolamine into phospholipids in germinating soya bean.

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1. Incorporation of [Me-14C]choline and [2-14C]ethanolamine into lipids was studied in germinating soya bean (Glycine max L.) seeds. The precursors are only incorporated into phosphatidylcholine and into phosphatidylethanolamine respectively. 2. Base-labelling via a phospholipase-D type of reaction

Action and Inhibition of Endogenous Phospholipases during Isolation of Plant Membranes.

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Endogenous phospholipase D and phosphatidic acid phosphatase activities were demonstrated in membrane fractions isolated from soybean (Glycine max L.) hypocotyls. Phospholipase D activity was distributed widely among different membrane fractions while phosphatidic acid phosphatase was found

Arsenic stress induces changes in lipid signalling and evokes the stomata closure in soybean.

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Soybean (Glycine max) is often exposed to high arsenic (As) level in soils or through irrigation with groundwater. In previous studies on As-treated soybean seedlings we showed deleterious effect on growth, structural alterations mainly in root vascular system and induction of antioxidant enzymes.

Transcriptomic Profiling of Soybean in Response to High-Intensity UV-B Irradiation Reveals Stress Defense Signaling.

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The depletion of the ozone layer in the stratosphere has led to a dramatic spike in ultraviolet B (UV-B) intensity and increased UV-B light levels. The direct absorption of high-intensity UV-B induces complex abiotic stresses in plants, including excessive light exposure, heat, and dehydration.

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