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pinus ayacahuite/agar
Veza se sprema u međuspremnik
2 rezultatima
In vitro regeneration of plantlets from mature embryos of Pinus ayacahuite.
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Prijava Registriraj se
A plantlet regeneration protocol was developed for Pinus ayacahuite var. ayacahuite (Ehrenb.). Embryos from mature seeds from ten provenances were cultured in a 16-h photoperiod for 3 days on a medium containing 30 mM sucrose and 0.7% agar. Cotyledons from these embryos were subcultured onto MCM
Root induction in Pinus ayacahuite by co-culture with Agrobacterium tumefaciens strains.
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Prijava Registriraj se
Transformation of in-vitro-derived shoots of Pinus ayacahuite Ehrenb. was achieved by co-culture with an oncogenic strain (A281 x 200) of Agrobacterium tumefaciens. During co-culture rooting also occurred; however, this rooting was not induced by genetic transformation of host cells, because a
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