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Biochimica et Biophysica Acta - General Subjects 1998-Feb

Molecular cloning and characterization of the gene encoding glutathione reductase in Brassica campestris.

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Abstrè

We have isolated the Brassica campestris cDNA encoding glutathione reductase of 502 amino acid residues with molecular mass of 54.5 kDa. The deduced amino acid sequences were 92.2%, and 79.5% identical to those of Arabidopsis thaliana, and pea, respectively. As expected, it exhibited a high degree of conservation within the region responsible for the redox reaction and for the binding of GSSG or NADPH. The gene was highly inducible by ozone fumigation or by paraquat treatment.

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