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Transgenic Research 2012-Feb

Transgenic production of arachidonic acid in oilseeds.

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James R Petrie
Pushkar Shrestha
Srinivas Belide
Maged P Mansour
Qing Liu
James Horne
Peter D Nichols
Surinder P Singh

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Abstrè

We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both the production of ARA in seed tissue and the low levels of intermediate C20 fatty acids that accumulate. We also demonstrate that the arachidonic acid is naturally enriched at the sn2 position in triacylglycerol. This is the first report of ARA production by the Δ9-elongase pathway in an oilseed.

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