amylase/glycine max

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Alpha-amylase from germinating soybean (Glycine max) seeds--purification, characterization and sequential similarity of conserved and catalytic amino acid residues.

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Starch hydrolyzing amylase from germinated soybeans seeds (Glycine max) has been purified 400-fold to electrophoretic homogeneity with a final specific activity of 384 units/mg. SDS-PAGE of the final preparation revealed a single protein band of 100 kDa, whereas molecular mass was determined to be

Safety evaluation of the food enzyme β-amylase obtained from soybean ( Glycine max)

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The food enzyme considered in this opinion is a β-amylase (EC 3.2.1.2) from soybean submitted by Nagase (Europa) GmbH. This β-amylase is intended to be used in the starch processing for maltose syrup production and the manufacture of a Japanese rice cake type. Based on the maximum use levels

Structural stability of soybean (Glycine max) α-amylase: properties of the unfolding transition studied with fluorescence and CD spectroscopy.

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Stability and unfolding of mammalian and microbial α-amylases have been intensively investigated. However, there is only limited information available on the structural stability of plant α-amylases, namely of the two isoenzymes from barley AMY1 and AMY2, of the α-amylase from mung bean (Vigna

Comparison of α-amylase, α-glucosidase and lipase inhibitory activity of the phenolic substances in two black legumes of different genera.

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Antioxidant-rich plant foods can inhibit starch and lipid digestions that are relevant to diabetes management. Two high-antioxidant black legumes, black soybean (Glycine max) and black turtle bean (Phaseolus vulgaris), belonging to two different genera were used to investigate their capacity against

Specific Determination of alpha-Amylase Activity in Crude Plant Extracts Containing beta-Amylase.

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The specific measurement of alpha-amylase activity in crude plant extracts is difficult because of the presence of beta-amylases which directly interfere with most assay methods. Methods compared in this study include heat treatment at 70 degrees C for 20 min, HgCl(2) treatment, and the use of the

Interactions of alpha-amylase and calcium chelator during neutral detergent fiber analysis.

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Amylase and calcium chelators, such as disodium ethylene diaminotetraacetate (EDTA), are used in analysis of neutral detergent fiber (NDF) to dissolve starch and pectin, respectively. However, these reagents may interfere with each other's activity. Six combinations of alpha-amylase and EDTA were

Influence of pulsed magnetic field on soybean (Glycine max L.) seed germination, seedling growth and soil microbial population.

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The effects of pulsed magnetic field (PMF) treatment of soybean (Glycine max L. cv CO3) seeds were investigated on rate of seed germination, seedling growth, physico-chemical properties of seed leachates and soil microbial population under laboratory conditions. Seeds were exposed to PMF of 1500 nT

The potential allergenicity of two 2S albumins from soybean (Glycine max): a protein microarray approach.

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BACKGROUND The 2S albumins are a group of storage proteins that occur widely in seeds of dicotyledonous plants. The widespread distribution and stability to digestion of allergenic 2S albumins raise the question of why some members of this family present in important food sources, such as soybean,

Antioxidant and antifungal activity of phenolic compounds and their relation with aflatoxin B1 occurrence in soybeans (Glycine max L.).

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BACKGROUND

Soybean is widely used in food formulations; however, few studies on fungal or mycotoxin contamination have been undertaken. In this regard, the free, conjugated and bound phenolic compounds, as well as their antioxidant and antifungal potential, besides the occurrence

Expanding resolution of metalloprotein separations from soybean seeds using 2D-HPLC-ICP-MS and SDS-PAGE as a third dimension.

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This work reports on the use of a three dimensional separation system to enhance metalloprotein information when considering soybean seeds. Separations using size exclusion chromatography (SEC) allowed identification of three metal fractions. Following an anion exchange (AEX) chromatographic

Composition and physical properties of starch in microgravity-grown plants.

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The effect of spaceflight on starch development in soybean (Glycine max L., BRIC-03) and potato (Solanum tuberosum, Astroculture-05) was compared with ground controls by biophysical and biochemical measurements. Starch grains from plants from both flights were on average 20-50% smaller in diameter

Molecular characterization of the diurnal/circadian expression of the chlorophyll a/b-binding proteins in leaves of tomato and other dicotyledonous and monocotyledonous plant species.

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Diurnal oscillations of steady-state mRNA levels encoding the chlorophyll a/b-binding proteins were monitored inLycopersicon esculentum, Glycine max, Phaseolus vulgaris, P. aureus, P. coccineus, Pisum sativum, Sinapis alba, Hordeum vulgare, Triticum aestivum andZea mays. In these plant speciescab

Affinity purification of trypsin inhibitor with anti-Aspergillus flavus activity from cultivated and wild soybean.

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Trypsin inhibitors (TI) from wild-type soybean (Glycine soya) (WBTI) and domesticated soybean (Glycine max) (SBTI) were purified using prepared chitosan resin-trypsin as filler on the affinity chromatography column. The SBTI/WBTI purification fold by affinity chromatography was 718- and 279-fold,

Short-term effect of dietary yeast nucleotide supplementation on total and diurnal variation of small intestinal enzyme activities in piglets.

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A study was carried out to investigate, whether short-term supplementation of dietary yeast nucleotides affects total and diurnal variation of enzyme activities in the small intestine of weaned piglets. Twelve barrows, weaned at 18 d of age (5 kg initial BW), were fitted with a simple T-cannula at

Involvement of nitrate reductase-dependent nitric oxide production in magnetopriming-induced salt tolerance in soybean.

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In the present study, experiments were performed to investigate the role of nitric oxide (NO) in magnetopriming-induced seed germination and early growth characteristics of soybean (Glycine max) seedlings under salt stress. The NO donor (sodium nitroprusside, SNP), NO scavenger

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