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A simple, sensitive HPLC method using fluorescence detection was developed for determination of adenosine in fetal venous perfusates of dual-perfused cotyledons from human term placentas. Maternal and fetal circuits of in vitro placental cotyledons were perfused with physiological salt solution
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BACKGROUND
This is a historical review of the method of "Ex vivo dual perfusion of a human placental cotyledon", which was first described by M. Panigel in 1967. The subsequent evolution of this method is described with particular emphasis on energy metabolism of human placental tissue under ex vivo
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