cotyledon/nicotine

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The effects of cocaine and nicotine on amino acid transport across the human placental cotyledon perfused in vitro.

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The inhibitory effects of cocaine and nicotine on placental amino acid transport, as a mechanism contributing to intrauterine growth restriction, were investigated in the in vitro placental perfusion model. Amino acids that represent substrates for known placental transporters were selected: alanine

High level expression of the Activator transposase gene inhibits the excision of Dissociation in tobacco cotyledons.

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A fusion of the strong cauliflower mosaic virus 35S promoter to the Activator (Ac) transposase (TPASE) gene does not trigger excision of Dissociation (Ds) continuously during tobacco cotyledon development, although once activated, the 35S promoter remains active throughout embryogeny. Epistasis

Promoter fusions to the Activator transposase gene cause distinct patterns of Dissociation excision in tobacco cotyledons.

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To explore the effects of altering the level of Activator (Ac) transposase (TPase) expression, a series of plasmids was constructed in which heterologous promoters were fused to the TPase gene. Promoters for the cauliflower mosaic virus (CaMV) 35S transcript and the octopine synthase (ocs) and

Modulation of the venation pattern of cotyledons of transgenic tobacco for the tumorigenic 6b gene of Agrobacterium tumefaciens AKE10.

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Neoplastic plant-tissue formation, termed crown gall disease, is induced on infection with Agrobacterium tumefaciens. The tumorous tissues develop an extensive vascular system, with a venation pattern distinct from that of native host plants. We report here that the plant-tumorigenic 6b gene of the

Evidence for type II phytochrome-induced rapid signalling leading to cab::luciferase gene expression in tobacco cotyledons.

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We studied the activation of cab gene expression by phytochrome-induced intercellular signalling and report insights into the mechanism of induction and outspread of a plant internal signal. By micro-beam irradiation techniques and use of a photon-imaging charge-coupled device (CCD) camera system we

Promoter and leader sequences of the spinach PsaD and PsaF genes direct an opposite light response in tobacco cotyledons: PsaD sequences downstream of the ATG codon are required for a positive light response.

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Subunits II and III of the photosystem I reaction centre are encoded by the nuclear genes PsaD and PsaF, respectively. In spinach, the expression of both genes is highly synchronized with regard to time, space and in response to stimulators such as light. Nevertheless, promoter sequences as well as

Nicotine-induced inhibition of lycopene cyclization in Phaseolus vulgaris cotyledons.

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The complete nicotine inhibition of lycopene cyclization during light-induced carotenogenesis in excised bean cotyledons was achieved. The inhibitory effect was easily reversible and removal of nicotine has allowed synthesis of the normal cyclic carotenoids.

Roles of extensins in cotyledon primordium formation and shoot apical meristem activity in Nicotiana tabacum.

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Extensins are cell wall basic glycoproteins with a polypeptide backbone that is extremely rich in hydroxyproline. In this paper, the function of extensins in embryo development was studied in Nicotiana tabacum. By using Western blot and immunohistochemistry, the extensin JIM20 epitopes were found to

Formation and retention of cotinine during placental transfer of nicotine in human placental cotyledon.

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Maternal smoking during pregnancy causes reduction of fetal breathing movements, an effect attributed to nicotine in fetal blood. Nicotine is metabolized to cotinine which has a long plasma half-life and exhibits slow clearance across membrane barriers. It is also known to activate placental

Transplacental transfer and biotransformation studies of nicotine in the human placental cotyledon perfused in vitro.

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Our objective was to study the characteristics of transfer and biotransformation of nicotine in the human term placenta. Nicotine transfer was studied by dually perfusing an isolated cotyledon of the human placenta in vitro. Nicotine metabolism to cotinine was investigated in intact tissue during

Tobacco cotyledons: a novel system for testing mutagenicity in plants.

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The effect of inoculum concentration on virus multiplication in cucumber cotyledons. II. Cucumber mosaic and tobacco mosaic viruses.

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Replication of tobacco ringspot virus. III. Site of virus synthesis in cucumber cotyledon cells.

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Effect of 2,4-dichlorophenoxyacetic Acid on the cytokinin requirement of soybean cotyledon and tobacco stem pith callus tissues.

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The Non-Mendelian Green Cotyledon Gene in Soybean Encodes a Small Subunit of Photosystem II.

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Chlorophyll degradation plays important roles in leaf senescence including regulation of degradation of chlorophyll-binding proteins. Although most genes encoding enzymes of the chlorophyll degradation pathway have been identified, the regulation of their activity has not been fully understood.

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