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Completely buried, non-ion-paired glutamic acid contributes favorably to the conformational stability of pyrrolidone carboxyl peptidases from hyperthermophiles.

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Pyrrolidone carboxyl peptidases (PCPs) from hyperthermophiles have a structurally conserved and completely buried Glu192 in the hydrophobic core; in contrast, the corresponding residue in the mesophile protein is a hydrophobic residue, Ile. Does the buried ionizable residue contribute to

[The effect of glutamic acid on the defensive and feeding behavior of the snail Helix lucorum].

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Behavioural effects of glutamic acid injection into the body cavity were investigated in the land snail Helix lucorum. An injection itself induced some non-coordinated defensive reactions leading to a partial withdrawal into a shell. The defensive tentacle reflex was facilitated probably due to an

A density functional and quantum Monte Carlo study of glutamic acid in vacuo and in a dielectric continuum medium.

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We present density functional theory (DFT) and quantum Monte Carlo (QMC) calculations of the glutamic acid and glutamate ion in vacuo and in various dielectric continuum media within the polarizable continuum model (PCM). In DFT, we employ the integral equation formalism variant of PCM while, in

Molecularly imprinted cryogel for L-glutamic acid separation.

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A molecular recognition based L-glutamic acid (L-GLU) imprinted cryogel was prepared for L-GLU separation via chromatographic applications. The novel functional monomer N-methacryloyl-(L)-glutamic acid-Fe(3+) (MAGA-Fe(3+) ) was synthesized to be complex with L-GLU. The L-GLU imprinted cryogel was

Laminarin Promotes Immune Responses and Normalizes Glutamic Oxaloacetic Transaminase and Glutamic Pyruvic Transaminase Levels in Leukemic Mice In Vivo.

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OBJECTIVE Laminarin, mainly found in the fronds of Laminaria, has antimicrobial characteristics and induces immune responses. However, there are no available information to show the laminarin effect on glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels in mice

Stabilizing role of glutamic acid 222 in the structure of Enhanced Green Fluorescent Protein.

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Enhanced Green Fluorescent Protein (EGFP) is a variant of wild-type Green Fluorescent Protein from the jellyfish Aequorea victoria, whose mutations S65T and F64L increase brightness and folding efficiency. EGFP is extensively used in cell biology and biochemistry as a colocalization or expression

Synthesis of cyclodextrin polymers containing glutamic acid and their use for the synthesis of Ag nanoparticles.

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Crosslinking and functionalizing agents play crucial roles in the properties/applications of cyclodextrin polymers. Here we report the synthesis of water-insoluble cyclodextrin polymers containing l-glutamic acid. In a first route, β-cyclodextrin was crosslinked with citric acid and subsequently

Evanescent wave cavity ring-down spectroscopy (EW-CRDS) as a probe of macromolecule adsorption kinetics at functionalized interfaces.

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Evanescent wave cavity ring-down spectroscopy (EW-CRDS) has been employed to study the interfacial adsorption kinetics of coumarin-tagged macromolecules onto a range of functionalized planar surfaces. Such studies are valuable in designing polymers for complex systems where the degree of interaction

Laminarin Promotes Immune Responses and Reduces Lactate Dehydrogenase But Increases Glutamic Pyruvic Transaminase in Normal Mice In Vivo.

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Laminarin, a typical component of fungal cell walls, has been shown to induce immune responses in both adult and larval locusts. We investigated the effects of laminarin on immune response and glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and lactate dehydrogenase

Dynamics of the glutamic acid 242 side chain in cytochrome c oxidase.

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In many cytochrome c oxidases glutamic acid 242 is required for proton transfer to the binuclear heme a(3)/Cu(B) site, and for proton pumping. When present, the side chain of Glu-242 is orientated "down" towards the proton-transferring D-pathway in all available crystal structures. A nonpolar cavity

Role of the intracellular cavity in potassium channel conductivity.

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The role of several fragments of the potassium channel KcsA has been examined by the Poisson-Nernst-Planck (PNP) theory. The efficiency of the computational method allowed comparing a large number of channel models, with different intracellular gate openings, partial atomic charges, and amino acid

Evanescent wave cavity ring-down spectroscopy as a probe of interfacial adsorption: interaction of Tris(2,2'-bipyridine)ruthenium(II) with silica surfaces and polyelectrolyte films.

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Evanescent wave cavity ring-down spectroscopy (EW-CRDS) has been used to study the interaction of the tris(2,2'-bipyridine)ruthenium(II) complex, [Ru(bpy)(3)](2+), at both native silica surfaces and surfaces modified with polyelectrolyte films. Both poly-l-lysine (PLL) and PLL/poly-l-glutamic acid

Analysis of surface cavity in serpin family reveals potential binding sites for chemical chaperone to reduce polymerization.

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Serpin constitute about 10% of blood protein and are associated with mutations that results in aberrant intermolecular linkages which leads to polymer formation. Studies with short peptides have shown promise in depolymerization of serpins however a reactive center loop based peptide also makes the

Chitosan promotes immune responses, ameliorates glutamic oxaloacetic transaminase and glutamic pyruvic transaminase, but enhances lactate dehydrogenase levels in normal mice in vivo.

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Chitosan, a naturally derived polymer, has been shown to possess antimicrobial and anti-inflammatory properties; however, little is known about the effect of chitosan on the immune responses and glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and lactate dehydrogenase

Intranasal immunization with poly(γ-glutamic acid) nanoparticles entrapping antigenic proteins can induce potent tumor immunity.

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We previously reported strong induction of ovalbumin (OVA)-specific tumor immunity in mice injected subcutaneously with OVA-entrapping nanoparticles comprising amphiphilic poly(γ-glutamic acid) (OVA/γ-PGA NPs). In the present study we investigated antitumor efficacy and associated immune responses

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