monogalactosyl diglyceride/epina

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Incorporation of fatty acids into monogalactosyl diglycerides by spinach leaf cell-free preparations.

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Incorporation of fatty acids into monogalactosyl diglyceride and acylation of monogalactosyl monoglyceride by spinach leaf preparations.

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Biosynthesis of galactolipids by enzyme preparations from spinach leaves.

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The pH optimum for galactolipid synthesis from UDP-galactose by spinach chloroplasts is 7.2 in Tris-HCl or phosphate buffer. The products include sterol glycosides, trigalactosyl diglyceride (tentatively identified), digalactosyl diglyceride, and monogalactosyl diglyceride in increasing order of

Role of Galactolipids in Spinach Chloroplast Lamellar Membranes: II. Effects of Galactolipid Depletion on Phosphorylation and Electron Flow.

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A galactolipid lipase from primary bean (Phaseolus vulgaris) leaves has been used to partially deplete spinach chloroplast inner membranes of their galactolipids. Chloroplasts treated with the lipase in the absence of bovine serum albumin lost 91% of their monogalactosyl diglyceride, 83% of their

Degradation of monogalactosyl diglyceride and digalactosyl diglyceride by sheep pancreatic enzymes.

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1. Saline extract of sheep pancreas acetone-dried powder was shown to catalyse acyl ester hydrolysis of spinach leaf galactosyl diglycerides and also galactosylglucosyl diglyceride of Lactobacillus casei. 2. Sodium deoxycholate stimulated the enzyme activity. Ca(2+) had no effect on the hydrolysis

The role of galactolipids in spinach chloroplast lamellar membranes: I. Partial purification of a bean leaf galactolipid lipase and its action on subchloroplast particles.

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A galactolipid lipase has been isolated and partially purified from the chloroplast fraction of the primary leaves of Phaseolus vulgaris var. Kentucky Wonder. The lipase hydrolyzed monogalactosyl diglyceride rapidly and phosphatidyl choline relatively slowly. Triolein and p-nitrophenyl stearate were

Differences in Lipid Composition between Undifferentiated and Mature Maize Chloroplasts.

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Lipid compositions of undifferentiated maize (Zea mays) chloroplasts, capable of fixing CO(2), were compared with the lipid compositions of mature chloroplasts, which do not fix CO(2), located in both the mesophyll and bundle sheath cells. The major lipids found in all three chloroplast types were

Localization of the tri- and digalactosyl diglyceride in the thylakoid membrane with serological methods.

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Trigalactosyl diglyceride was isolated from leaves of Urtica dioica and characterized by thin layer chromatography, infrared spectroscopy and by its fatty acid composition. An antiserum to the trigalactolipid was obtained by immunization of rabbits. By means of inhibition experiments with

Inhibition of glycolipid biosynthesis in chloroplasts by ozone and sulfhydryl reagents.

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The metabolism of uridine 5'-pyrophosphate-galactose by spinach (Spinacia oleracea) chloroplast preparations was inhibited by ozone. The formation of digalactosyl diglyceride and trigalactosyl diglyceride was inhibited much more than the formation of monogalactosyl diglyceride, steryl glycoside, and

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