p benzoquinone/epina

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The effect of various substituted p-benzoquinones on electron transport in spinach chloroplasts.

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Inhibition of photosynthetic electron transport in isolated spinach chloroplasts by two 1,3,4-thiadiazolyl derivatives.

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Buthidazole (3-[5-(1,1-dimethylethyl)-1,3,4-thiadiazol-2-yl]-4-hydroxy-1-methyl-2-imidazolidinone) and tebuthiuron (N-[5-(1,1-dimethylethyl)-1,3,4-thiadiazol-2-yl]-N,N'-dimethylurea) are two new promising herbicides for selective weed control in corn (Zea mays L.) and sugarcane (Saccharum

Cytochrome b-563 redox changes in intact CO-fixing spinach chloroplasts and in developing pea chloroplasts.

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Intact spinach chloroplasts, capable of high rates of photochemical oxygen evolution with CO2 as electron acceptor (120-350 mumol O2 mg chlorophyll-1 h-1) were examined for cytochrome redox changes. The response of the cytochromes in intact chloroplasts to oxidants and reductants appears to be

Oxidation-reduction coupled phosphorylation in the dark with isolated spinach chloroplasts.

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1. Spinach chloroplasts, pre-incubated with ferricyanide, acquire the ability to make ATP in the dark provided they are supplied with a reductant and a lipophilic mediator that can penetrate the membrane. The mediator must be of the type that, upon oxidation, releases protons into the surrounding

Adenylate regulation of photosynthetic electron transport and the coupling sites of phosphorylation in spinach chloroplasts.

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The regulation by adenylates of activities of various partial electron transport systems in spinach chloroplasts was studied using systems from H2O to 2,5-dimethyl-p-benzoquinone, H2O to 2,6-dichlorophenolindophenol, reduced 2,6-dichlorophenolindophenol to methyl viologen, and H2O to methyl viologen

Effect of osmotic stress on photosynthesis studied with the isolated spinach chloroplast : generation and use of reducing power.

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The effect of increasing assay medium sorbitol concentration from 0.33 to 1.0 molar on the photosynthetic reactions of intact and broken spinach (Spinacia oleracea L. var. Long Standing Bloomsdale) chloroplasts was investigated by monitoring O(2) evolution supported by the addition of glyceric acid

Chlorophyll a fluorescence measurements of isolated spinach thylakoids obtained by using single-laser-based flow cytometry.

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Flow cytometry data of spinach thylakoid membrane preparations indicate the presence of a homogeneous thylakoid population. Fluorescence data from a flow cytometer and comparison with data from two other fluorometers show that chlorophyll a fluorescence detected with a flow cytometer has the

Characterization of an Electron Transport Pathway Associated with Glucose and Fructose Respiration in the Intact Chloroplasts of Chlamydomonas reinhardtii and Spinach.

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The role of an electron transport pathway associated with aerobic carbohydrate degradation in isolated, intact chloroplasts was evaluated. This was accomplished by monitoring the evolution of (14)CO(2) from darkened spinach (Spinacia oleracea) and Chlamydomonas reinhardtii chloroplasts externally

Organization of Electron Transport in Photosystem II of Spinach Chloroplasts According to Chelator Inhibition Sites.

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The organization of electron transport in photosystem II of spinach (Spinacia oleracea) chloroplasts was studied by means of various chelators and uncouplers. The partial reactions used included H(2)O-->methyl viologen, H(2)O-->silicomolybdic acid H(2)O-->ferricyanide, and

A cytochrome f/b6 complex of five polypeptides with plastoquinol-plastocyanin-oxidoreductase activity from spinach chloroplasts.

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The isolation of a cytochrome f/b6 complex from spinach chloroplasts, with high yield and purity is reported. The complex consists of five polypeptides with a molecular mass of 34, 33, 23.5, 20 and 17.5 kDa, and contains one cytochrome f, two cytochromes b6 and the Rieske Fe-S center with two

Isolation of photosystem II enriched membrane vesicles from spinach chloroplasts by phase partition.

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Partition in an aqueous Dextran-polyethylene glycol two-phase system has been used for the separation of chloroplast membrane vesicles obtained by press treatment of a grana-enriched fraction after unstacking in a low salt buffer. The fractions obtained were analysed with respect to chlorophyll,

Inside-out membrane vesicles isolated from spinach thylakoids.

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Inside-out thylakoid vesicles have been separated from right-side-out material after press disruption of chloroplast lamellae. The sepration was obtained by partitionin an aqueous dextran-polyethylene glycol two-phase system, a method which utilizes differences in surface properties for separation

Antioxidant activity of reduced plastoquinone in chloroplast thylakoid membranes.

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The antioxidant effect of reduced plastoquinone was studied in chloroplast membranes. Isolated spinach thylakoid membranes were subjected to strong illumination followed by analysis of pigment bleaching and lipid peroxidation. The plastoquinone pool was kept in the reduced or oxidized state during

Functional size of Photosystem II determined by radiation inactivation.

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The functional size of Photosystem II (PS II) was investigated by radiation inactivation. The technique provides an estimate of the functional mass required for a specific reaction and depends on irradiating samples with high energy γ-rays and assaying the remaining activity. The analysis is based

Characterization of electron transfer from water to plastocyanin catalyzed by resolved electron transfer complexes from chloroplasts.

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The photoreduction of soluble plastocyanin in a homologous reconstituted system requires a resolved Photosystem II preparation and the cytochrome b6-f complex from spinach chloroplasts. Oxygen evolution is linked quantitatively to plastocyanin reduction in the reconstituted system, indicating that

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