purine/nicotiana

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Purine metabolism in mesophyll protoplasts of tobacco (Nicotiana tabacum) leaves.

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The overall metabolism of purines was studied in tobacco (Nicotiana tabacum) mesophyll protoplasts. Metabolic pathways were studied by measuring the conversion of radioactive adenine, adenosine, hypoxanthine and guanine into purine ribonucleotides, ribonucleosides, bases and nucleic acid

Effect of allopurinol on the utilization of purine degradation pathway intermediates by tobacco cell cultures.

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Suspension cultured Nicotiana tabacum (tobacco) cells grow slowly on intermediates of the purine degradation pathway (hypoxanthine, xanthine, uric acid, allantoin, and urea) as their sole nitrogen source indicating that this degradation pathway is operative in these cells. The hypoxanthine analog,

Purification, immunoassay and characterization of an abundant, cytokinin-regulated polypeptide in cultured tobacco tissues : Evidence the protein is a β-1,3-glucanase.

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We have purified an abundant, 33000-dalton polypeptide (P33) from cultured pith parenchyma tissue of Nicotiana tabacum L. cv. Havana 425. The accumulation of P33 in culture is inhibited by the cytokinin kinetin (N(6)-furfuryl-amino purine). When tissues are subcultured on auxin-containing medium,

The CRK1 receptor-like kinase gene of tobacco is negatively regulated by cytokinin.

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This report describes a novel receptor-like kinase gene of tobacco (Nicotiana tabacum L.) that, in cell culture, is rapidly regulated by very low concentrations of cytokinin. The steady-state transcript level of the CYTOKONIN-REGULATED KINASE 1 gene (CRK1) was strongly reduced 30 min after cytokinin

The mode of incorporation of 6-benzylaminopurine into tobacco callus transfer ribonucleic Acid: a double labeling determination.

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Cytokinin-dependent tobacco (Nicotiana tabacum) tissue was grown in the presence of N(6)-benzyladenine (6-benzylaminopurine) labeled in the phenyl ring with (3)H and in the 8-position of the purine ring with (14)C. The (3)H/(14)C ratio of N(6)-benzyladenosine recovered from labeled tobacco callus

Mode of action of N,N'-diphenylurea: The isolation and identification of the cytokinins in the transfer RNA from tobacco callus grown in the presence of N,N'-diphenylurea.

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The tRNA from cytokinin-dependent tobacco callus (Nicotiana tabacum) grown on mineral medium containing N,N'-diphenylurea as the source of cytokinin was found to contain 3 cytokinin-active ribonucleosides. The 2 ribonucleosides present in the largest amounts were identified conclusively by their

Two effects of cytokinin on the auxin requirement of tobacco callus cultures.

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Cytokinin affects the requirement for auxin of a strain of tobacco callus (Nicotiana tabacum) which is cytokinin-autotrophic when grown on Murashige and Skoog medium with 11.4 mum of indole-3-acetic acid but requires cytokinin 6-(3-methyl-2-butenylamino)purine (i(6) Ade) when grown on the same

Modification of disease resistance of tobacco callus tissues by cytokinins.

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The effects of differing cytokinin and auxin concentrations on resistance of tobacco (Nicotiana tabacum L.) tissue cultures to race 0 of Phytophthora parasitica var. nicotianae were examined. With 1 micromolar kinetin and either 11.5 micromolar indoleacetic acid or 1 micromolar

Cytokinin biosynthesis in cultured rootless tobacco plants.

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Biosynthesis of cytokinin in shoots was examined by growing rootless tobacco (Nicotiana tabacum) plants in vitro. The rootless plants were originated by culturing tobacco callus on a high cytokinin-low auxin medium to induce the formation of plantlets which were then grown on medium without

Tobacco NUP1 transports both tobacco alkaloids and vitamin B6.

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The purine permeases (PUPs) constitute a large plasma membrane-localized transporter family in plants that mediates the proton-coupled uptake of nucleotide bases and their derivatives, such as adenine, cytokinins, and caffeine. A Nicotiana tabacum (tobacco) PUP-family transporter, nicotine uptake

Molecular and functional characterization of allantoate amidohydrolase from Phaseolus vulgaris.

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Allantoate degradation is an essential step for recycling purine-ring nitrogen in all plants, but especially in tropical legumes where the ureides allantoate and allantoin are the main compounds used to store and transport the nitrogen fixed in nodules. Two enzymes, allantoate amidohydrolase (AAH)

Co-Suppression of NbClpC1 and NbClpC2, Encoding Clp Protease Chaperons, Elicits Significant Changes in the Metabolic Profile of Nicotiana benthamiana.

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Metabolites in plants are the products of cellular metabolic processes, and their differential amount can be regarded as the final responses of plants to genetic, epigenetic, or environmental stresses. The Clp protease complex, composed of the chaperonic parts and degradation proteases, is the major

Translocation and accumulation of nicotine via distinct spatio-temporal regulation of nicotine transporters in Nicotiana tabacum.

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In plants, secondary metabolites play important roles in adaptation to the environment. Nicotine, a pyridine alkaloid in Nicotiana tabacum, functions as chemical barrier against herbivores. Nicotine produced in the root undergoes long-distance transport and accumulates mainly in the leaves. Since

The influence of genomes on autonomous growth of pith cultures of Nicotiana glauca-Langsdorffii hybrids.

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A differential influence of the two parental genomes on cell proliferation and morphogenesis in pith tissue explants can be observed among the various tumorous hybrid combinations between Nicotiana glauca Grah. and N. langsdorffii Weinm.: the F1 hybrid (GL), its amphiploid (GGLL), and two different

Cytokinins: synthesis and biological activity of geometric and position isomers of zeatin.

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Geometric and position isomers of zeatin and of ribosylzeatin and other compounds closely related to zeatin have been tested in the tobacco (Nicotiana tabacum var. Wisconsin No. 38) bioassay. None was more active than zeatin itself. There was a much greater difference in activity (> 50-fold) between

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