Bioavailability of Protein and Amino Acids From Oilseeds in Healthy Volunteers
Kulcsszavak
Absztrakt
Leírás
4 groups of healthy volunteers are recruited (males and females from 18 to 65 y old), BMI from 18 to 30.
Each group tests a protein source (20g) incorporated in a biscuit that is divided in small portions to perform a repeated meal protocol. Protein sources (sunflower, rapeseed, flaxseed or lupin) are intrinsically labeled with 15N and deuterium (2H).
The day before the meal test, the volunteers are equipped with an intestinal tube that migrates until the ileum. On the day of the experiment, the position of the intestinal tube is checked by radiography in order to verify its location at the terminal ileum. A catheter is inserted in the forearm vein for blood sampling. A saline solution containing polyethylene glycol 4000 (PEG-4000, 20 g/L), used as a non-absorbable marker of the intestinal flow, is infused into the ileum in order to calculate the flow rate of the intestinal effluents. After a basal blood and urine sampling, as well as a basal collection of ileal effluents performed for 30 min, subject ingests at t=0 the first biscuit dose. The small biscuit portions are ingested every 30 min for 4h, to achieve an isotopic plateau. Tracer doses of carbon 13 (13C) amino acids are ingested concomitantly with the biscuit doses. Non absorbable markers are added to the test meal to correct for incomplete recovery at the ileal level.
The postprandial sampling period lasts for 8 h after the meal ingestion. The intestinal content is continuously collected over ice and pooled every 30 min. Blood is sampled every 30 min for 4 h, and hourly thereafter.Total urine is collected every 2 h.
Measurements:
In the effluents, the investigators measure PEG 4000, nitrogen (N) and 15N, carbon and 13C, amino acids (AA), 15N and 13C in amino acids and the non-absorbable markers of the meal. The investigators calculate the ileal flow rate, the overall real ileal protein digestibility and the real ileal digestibility of individual amino acids.
In the plasma, the investigators measure uremia and 15N in urea, AA and protein to measure the transfer of dietary N in plasma protein pools; 15N, 2H and 13C in individual amino acids too measure the relative bioavailability of 15N/2H amino acids compared to free 13C amino acids.
In the urine, the investigators measure urea and 15N in the urea ammonia to calculate the postprandial deamination losses.
These measurements will allow to determine the protein and amino acid bioavailability from 4 different plant protein sources, using the classical method implying ileal sampling and intrinsic tracer of dietary protein (15N). Additionally, it will allow to test a less invasive procedure using multiple tracers to assess the amino acid bioavailability.
Dátumok
| Utolsó ellenőrzés: | 08/31/2019 |
| Első benyújtás: | 02/18/2019 |
| Becsült beiratkozás benyújtva: | 07/15/2019 |
| Első közzététel: | 07/17/2019 |
| Utolsó frissítés beküldve: | 09/18/2019 |
| Utolsó frissítés közzétéve: | 09/19/2019 |
| A tanulmány tényleges kezdési dátuma: | 01/14/2019 |
| Becsült elsődleges befejezési dátum: | 12/29/2020 |
| A tanulmány becsült befejezési dátuma: | 03/29/2021 |
Állapot vagy betegség
Beavatkozás / kezelés
Dietary Supplement: Sunflower
Dietary Supplement: Rapeseed
Dietary Supplement: Flaxseed
Dietary Supplement: Lupin
Fázis
Karcsoportok
| Kar | Beavatkozás / kezelés |
|---|---|
| Experimental: Sunflower Biscuits containing sunflower isolate intrinsically labelled with 15N and 2H | Dietary Supplement: Sunflower Plant proteins are intrinsically enriched with 15N and 2H. There are incorporated into biscuits. The biscuits are formulated to supply 20 g of plant protein per volunteer. |
| Experimental: Rapeseed Biscuits containing rapeseed isolate intrinsically labelled with 15N and 2H | Dietary Supplement: Rapeseed Plant proteins are intrinsically enriched with 15N and 2H. There are incorporated into biscuits. The biscuits are formulated to supply 20 g of plant protein per volunteer. |
| Experimental: Flaxseed Biscuits containing flaxseed isolate intrinsically labelled with 15N and 2H | Dietary Supplement: Flaxseed Plant proteins are intrinsically enriched with 15N and 2H. There are incorporated into biscuits. The biscuits are formulated to supply 20 g of plant protein per volunteer. |
| Experimental: Lupin Biscuits containing lupin flour intrinsically labelled with 15N and 2H | Dietary Supplement: Lupin Plant proteins are intrinsically enriched with 15N and 2H. There are incorporated into biscuits. The biscuits are formulated to supply 20 g of plant protein per volunteer. |
Jogosultsági kritériumok
| Tanulásra alkalmas korok | 18 Years Nak nek 18 Years |
| Tanulásra alkalmas nemek | All |
| Egészséges önkénteseket fogad | Igen |
| Kritériumok | Inclusion Criteria: - 18 - Healthy - Insured under the French social security system - for women: use of birth control - signed informed consent Exclusion Criteria: - Any dietary allergy - Latex allergy - Positive serology to HIV, hepatite C virus antibody, hepatite B virus surface antigen and core antibodies - Gluten intolerance - Anemia - Abusive use of drugs or alcohol - Hypertension, diabetes, digestive disease, hepatic or renal disease, severe cardiac disease - Pregnancy - High sport practicing (>7h/wk) - Blood donation in the 3 months prior to the study - Participation in a clinical study in the 3 months prior to the study - No signed informed consent |
Eredmény
Elsődleges eredménymérők
1. Percentage of ileal digestibility of plant proteins [time -30 minutes to 8 hours after the meal]
2. Percentage of ileal digestibility of amino acids from plant proteins [time -30 minutes to 8 hours after the meal]
3. Percentage of net postprandial protein utilization of plant proteins [time -30 minutes to 8 hours after the meal]
4. Postprandial plasma kinetics of dietary amino acids [time -30 minutes to 8 hours after the meal]
5. Postprandial metabolism of glucose 1 [time -30 minutes to 8 hours after the meal]
6. Postprandial metabolism of glucose 2 [time -30 minutes to 8 hours after the meal]
Másodlagos eredménymérők
1. Validation of the dual isotope method to assess the bioavailability of individual amino acids [time -30 minutes to 8 hours after the meal]
