Anti-inflammatory evaluation and acute toxicity of three food supplements that contain Moussonia deppeana.

OBJECTIVE

To identify the anti-inflammatory activity through two murine models and in the median Lethal Dose (LD50) of three dietary supplements that contain Moussonia deppeana.

METHODS

The anti-inflammatory activity of three dietary supplements (Cicatrisan/Gastricus®, Gastinol®, and Gastrovita®) EtOH extracts was evaluated by TPA and by carrageenan murine models; also, median Lethal Dose (LD50) was determined. Verbascoside was quantified by High-Performance Liquid Chromatography. β-sitosterol, stigmasterol and the mixture of ursolic and oleanolic acids were identified in all supplements by TLC; however, none of these dietary supplements contain verbascoside.

RESULTS

For the TPA model, Cicatrisan/Gastricus® generated a notable effect with 38.24% inhibition. While in the carrageenan model, it also exhibited noteworthy anti-inflammatory activity of ear edema with 66.39% of paw edema inhibition at 150 mg/kg, followed by Gastinol® and Gastrovita® with ≈50% at 300 mg/kg. Finally, LD50 was >2 g/kg for all supplements, when was administered intragastrically and Body Weight (BW) gain in mice was not altered after 14 days.

CONCLUSIONS

Of the three food supplements containing M. deppeana, only the EtOH extract from Cicatrisan/Gastricus® formulation (tablets) showed significant anti-inflammatory activity in both experimental models and the LD50 was >2 g/kg.