[Chemical constituents analysis on anticonvulsive effect of three extracts from radix bupleuri].

OBJECTIVE

To study the relationship between anticonvulsive effect of three extracts from Radix Bupleuri and their chemical compositions.

METHODS

Volatile oil component, saikosaponin component and water-soluble component were extracted with SFE-CO2 from Radix Bupleuri, their effect on MES and PTZ seizure models had been studied, and their compositions were analyzed by GC-MS and HPLC.

RESULTS

Compared with the control, volatile oil component and saikosaponin component significantly decreased anticonvulsive occurrence on MES, the main chemical compositions of them was linoleic acid; water-soluble component significantly decreased anticonvulsive occurrence on PTZ seizure, but most of its compositions were glycerol and xylitol.

CONCLUSIONS

Linoleic acid from Radix Bupleuri maybe fight against MES seizure, while glycerol and xylitol could fight against PTZ seizure.