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Dielectric dynamic behavior of bovine serum albumin (BSA)-water mixtures over wide ranges of water fractions, from dry protein until 40 wt % in water, was studied through dielectric relaxation spectroscopy (DRS). The α relaxation associated with the glass transition of the hydrated system was
Novel multicomponent organogels containing an aqueous phase are described, and some properties which influence their potential as delivery devices for hydrophilic drugs and vaccines are discussed. The gel is produced by preparing a hot water-in-oil (w/o) emulsion using sorbitan monostearate, a
The previously advanced hypothesis that desiccation resistance involves the replacement of water adjacent to intracellular surfaces with polyhydroxy compounds has been supported by experiments on cysts of the brine shrimp, Artemia, and in a model system of albumin--glycerol--water, using nuclear
By recognizing the forces that drive water transport across cell membranes or across tissues, we can see how water is driven to and from proteins. We learn from examples. When a dissolved protein (bovine serum albumin) accumulates water relative to small solutes, it effectively withdraws a number of
Thermodynamic incompatibility and two-dimensional phase separation in a bovine serum albumin (BSA)/beta-casein/water ternary film at the air-water interface has been studied using an epifluorescence microscopy technique. The incompatibility between BSA and beta-casein at the air-water interface was
Protein-encapsulated nanoclusters (NCs) are emerging as a versatile platform for in-vivo imaging and other biomedical applications due to their ultra-small size and excitation in the near-infrared. Encapsulation may however affect protein structure, its size, charge, and interaction with lipid
The movement of water between the air space and capillary compartments is important for the maintenance of air space hydration during respiration and for reabsorption of excess alveolar fluid. We have obtained immunocytochemical and functional evidence that plasma-membrane water channels are
OBJECTIVE
In this study, we developed and evaluated a novel method to produce insulin-loaded hydrophilic microspheres allowing high encapsulation efficiency and the preservation of peptide stability during particle processing. The preparation method used the diffusion of water by an excess of
Protein-loaded starch microspheres were prepared by water-in-water (w/w) emulsion method. The effects of the molecular weight of starch and protein used, concentration of solutes in both dispersed and continuous phases and starch to protein mass ratio on the yield, loading capacity and encapsulation
A model water-in-oil-in-water (W1/O/W2) double emulsion was prepared by a two-step emulsification procedure and subsequently subjected to temperature changes that caused the oil phase to freeze and thaw while the two aqueous phases remained liquid. Our previous work on individual double-emulsion
A rapid, controlled microwave oven thawing technic for fresh frozen plasma (FFP) that minimizes flocculent formation while maintaining excellent coagulation factor, total protein, and albumin levels is presented. This method, using an unmodified microwave oven and a tap-water environment, decreases