Polypod-like structured guanine-rich oligonucleotide aptamer as a selective and cytotoxic nanostructured DNA to cancer cells
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Guanine-rich oligonucleotide (GRO) can be developed as an effective anticancer agent owing to its high selectivity, affinity, and antiproliferative activity in cancer cells. In this study, to increase the potency of GRO29A, a 29-mer GRO aptamer against nucleolin, an overexpressed protein in cancer cells, GRO29A was incorporated into three or six pods of polypod-like structured DNA (polypodna), tripodna or hexapodna, respectively. The polypod-like structured GROs, tri-G3, consisting of one tripodna and three GRO29A, or hexa-G1, hexa-G3, or hexa-G6, each of which comprises one hexapodna and one, three, or six GRO29A, respectively, were designed. Tri-G3, hexa-G1, and hexa-G3 were prepared in high yield, except for hexa-G6. Polypod-like structured GROs had quadruplex structures under physiological salt condition, and degraded at a slower rate in buffer containing serum. Cellular interaction experiments using
Keywords: DNA aptamer; Polypod-like structured DNA; cancer cell; guanine-rich oligodeoxynucleotide; nucleolin; self-assembly.