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The subcellular distribution of the phospholipase activities and metabolism of lysophosphatidylcholine in cultured human cell line (FL cells) during "fusion-from-within" induced by measles virus were studied. During cell fusion, fairly high activities of phospholipase A1 and A2, the optimal pHs of
Change of lipid metabolism in FL cells derived from human amniotic membrane during fusion from within induced by measles virus was studied. The following results were obtained. (1) Incorporation of [32P]orthophosphate and [methyl]-14C]choline into the lipid fraction of infected cells was higher than
We found that nontoxic doses of two inhibitors of cholesterol synthesis, namely W-7 and cerulenin, delayed syncytia formation in vero cells infected with measles virus. To correlate syncytia formation and lipidic membrane changes induced by these drugs, we labelled cell lipids with [14C]acetate.
In BGM cells chronically infected with measles virus, although the composition of the phospholipids is unaltered, the fatty acid composition is modified. Uninfected, lytic and persistently infected cells were labelled with [3H]arachidonic acid and [14C]stearic acid and their metabolic fate analysed.
We have examined whether the passage of a paramyxovirus in a cell (BGM, African green monkey kidney) or animal (Swiss mouse) can permanently modify its metabolism. In an in vitro model in which cells had been cured of a measles virus persistent infection, the cells retained the modifications