A new concept in hybridization: Bromelain enzyme for deproteinizing dentin before application of adhesive system.
Kata kunci
Abstrak
OBJECTIVE
The objective of this study is to assess the deproteinizing effect of bromelain enzyme and compare it with neodymium-doped yttrium aluminum garnet (Nd:YAG) laser and 10% sodium hypochlorite (NaOCl) by using scanning electron microscope (SEM) and polarized microscope.
METHODS
A total of 60 extracted human upper premolars were selected to be given standardized buccal and lingual class V cavities. The teeth were divided into three groups each one consisted of 20 teeth. Thirty teeth were recruited for SEM study and the other 30 for polarized microscope. Group 1: Teeth were deproteinized with Nd:YAG laser, Group 2: Teeth were deproteinized with bromelain enzyme and Group 3: Teeth were deproteinized with 10% NaOCl.
CONCLUSIONS
Application of bromelain enzyme has led to removing collagen network and significantly decreased the global leakage scores of the adhesive system.
