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Multiple CH/π Interactions Maintain the Binding of Aflatoxin B₁ in the Active Cavity of Human Cytochrome P450 1A2.

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Human cytochrome P450 1A2 (CYP1A2) is one of the key CYPs that activate aflatoxin B₁ (AFB₁), a notorious mycotoxin, into carcinogenic exo-8,9-epoxides (AFBO) in the liver. Although the structure of CYP1A2 is available, the mechanism of CYP1A2-specific binding to AFB₁ has not been fully clarified. In

Ultrasound assisted combined molecularly imprinted polymer for the selective micro-solid phase extraction and determination of aflatoxins in fish feed using liquid chromatography-tandem mass spectrometry.

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A combined procedure based on using ultrasounds for target isolation followed by porous membrane-protected micro solid phase extraction using a molecularly imprinted polymer as an adsorbent has been developed as a highly selective extraction and clean-up procedure for isolating aflatoxins B1, B2,

Comparative evaluation of aflatoxin and mineral binding activity of molecular imprinted polymer designed for dummy template using in vitro and in vivo models.

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The amelioration of aflatoxicosis in ducklings was examined by feeding molecular imprinted polymer (MIP) synthesized to target the aflatoxin B₁ (AFB₁) analog molecule [5, 7-dimethoxycoumarin (DMC)] as a smart and novel toxin binder in comparison to a commercial toxin binder

Effect of lifetime exposure to aflatoxin b1 in rats.

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Aflatoxin B1 was fed at 2 ppm in the diet to a group of pregnant F344 rats from the time of conception; it was then fed to their offspring until death. This diet was also given to another group of rats 6-7 weeks old for comparison. The survival time of male rats was significantly shorter than that

Aflatoxin B1 Detection Using a Highly-Sensitive Molecularly-Imprinted Electrochemical Sensor Based on an Electropolymerized Metal Organic Framework.

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A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of

Isolation of a metastasizing cancer cell line from an aflatoxin B1-induced rat liver tumor.

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An attempt was made to isolate cancer cell lines from liver tumors that had been induced by aflatoxin B1 (AFB1) in rats. A clonal cell line named AFB-1 was isolated from a liver tumor that was histologically diagnosed as hepatocellular carcinoma. When AFB-1 cells were inoculated into the

Room temperature phosphorescent determination of aflatoxins in fish feed based on molecularly imprinted polymer - Mn-doped ZnS quantum dots.

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Possibilities of room temperature spectrometry based on Mn-doped ZnS quantum dots coated with a molecularly imprinted polymer based nanosensor have been explored for the sensitive and selective determination of aflatoxins. Synthesized polymeric nanoparticles exhibit intense room temperature

Electrochemical aptasensor for aflatoxin B1 based on smart host-guest recognition of β-cyclodextrin polymer.

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Developing a simple and reliable method for the detection of the highly concerning mycotoxin, aflatoxin B1 (AFB1), is of great importance to food safety monitoring. In this study, a simple electrochemical aptasensor was presented for the detection of AFB1 based on the host-guest recognition between

Influence of P450 3A4 SRS-2 residues on cooperativity and/or regioselectivity of aflatoxin B(1) oxidation.

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The major human liver drug-metabolizing cytochrome P450 enzymes P450 3A4 and P450 3A5 share >85% amino acid sequence identity yet exhibit different regioselectivity toward aflatoxin B(1) (AFB(1)) biotransformation [Gillam et al. (1995) Arch. Biochem. Biophys. 317, 74-384]. P450 3A4 prefers AFB1

Aluminosilicate clay improves production responses and reduces inflammation during an aflatoxin challenge in lactating Holstein cows.

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Mitigation strategies are vital in minimizing the health and economic risks associated with dairy cattle exposure to aflatoxin (AF). The objective of this study was to determine the effects of a commercially available aluminosilicate clay in a lactation diet on production responses, blood chemistry,

Effects of clay after an aflatoxin challenge on aflatoxin clearance, milk production, and metabolism of Holstein cows.

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Oral supplementation of clay to dairy cattle has been reported to reduce toxicity of aflatoxin (AF) in contaminated feed. The objective of this study was to determine the effects of 3 concentrations of dietary clay supplementation in response to an AF challenge. Ten multiparous rumen-cannulated

The short-term effect of low concentrations of dietary aflatoxin and T-2 toxin on mallard ducklings.

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Two 9-day trials to study effects of dietary mycotoxins were conducted using 6-week-old mallards. Each trial involved three groups of six ducks each, three males and three females per group. One group was fed no myocotoxin (control), one was fed aflatoxin, and the third was fed T-2 toxin. Dietary

Preparation and characterization of yeast cell wall beta-glucan encapsulated humic acid nanoparticles as an enhanced aflatoxin B1 binder.

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This study aimed to assess the effect of encapsulating humic acid inside yeast cell walls (YCW) to detoxify AFB1 in in vitro gastrointestinal models. Glucan Mannan Lipid Particles (GMLPs) from Saccharomyces cerevisiae cell walls showed the highest AFB1 adsorption in simulated gastric fluid (SGF)

Structure, function, regulation and polymorphism of human cytochrome P450 2A6.

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The CYP2A6 gene spans a region of approximately 6 kb pairs consisting of 9 exons and has been mapped to the long arm of chromosome 19 (between 19q12 and 19q13.2). The CYP2A6 protein has 494 amino acids and is an important hepatic Phase I enzyme that metabolizes approximately 3% of therapeutic drugs

[Dietary factor and cancer risk--evidence from epidemiological studies].

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Dietary factors including obesity and physical activity are estimated to account for approximately 35% of cancer death in the United States. According to the WHO/FAO report in 2003 based on a review of published epidemiological studies, convincing evidence between diet-related factors and cancer are

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