Oral soft tissues biopsies (alveolar mucosa, buccal gingiva, and palatal tissue) at T0 and T24

Wound Heal

Wound Surgical

Oral Soft Tissue Conditions

Chlorhexidine Adverse Reaction

Oral soft tissues biopsies (alveolar mucosa, buccal gingiva, and palatal tissue) will be harvested by the examiner at the time of the surgery (immediately before to start the surgical procedure -T0) and 24 hours after surgery (T24)-after chlorhexidine use- at the level of the vertical released incisions (VRIs) with a biopsy punch with plunger of 2.0 mm diameter.

Oral soft tissues biopsies

Department of Oral and Maxillofacial Sciences. Section of Periodontics.Sapienza, University of Rome

Early Healing of Oral Soft Tissues: a Clinical and Biomolecular Analysis. Part II

Early Healing of Oral Soft Tissues: a Clinical and Biomolecular Analysis. Part II - Effect of Local Chlorhexidine Administration in Gene Expression and Cellular Behaviour 24-hours After Injury

Total RNA from biopsies or cell cultures was extracted using TRIzol reagent Quantitative real-time PCR (qRT-PCR) cDNA was generated and cDNA obtained were used for amplification of wound healing related genes using the appropriate TaqMan gene expression assay kits.

Assessed with a clinical index (EHS- Early wound healing score). This score assessed clinical signs of re-epithelialization (CSR), clinical signs of haemostasis (CSH), and clinical signs of inflammation (CSI). Since complete wound epithelialization was the main outcome, the CSR score was weighted to be 60% of the total final score. Accordingly, a score of 0, 3, or 6 points was possible for the assessment of CSR, whereas scores of 0, 1, or 2 points were possible for CSH and CSI. Higher values indicated better healing. Accordingly, the score for ideal early wound healing was 10.

Andrea Pilloni MD DDS MS

Chairman Section of Periodontics Director of Master Program in Periodontics

University of Roma La Sapienza

in diabetic patients, the subcutaneous fat will be closed with three to five interrupted sutures. The sutures were tied until the tissue was adequately re-approximated, but not as hard as possible to avoid necrosis after cesarean section.

in diabetic patients, the subcutaneous fat will not sutured after cesarean section.

Abdominal Delivery

the subcutaneous fat will be closed with three to five interrupted sutures. The sutures were tied until the tissue was adequately re-approximated, but not as hard as possible to avoid necrosis after cesarean section in diabetic patients.

closure of subcutaneous tissue

the subcutaneous fat will not sutured after cesarean section in diabetic patients.

No closure of subcutaneous tissue

Closure Versus Non Closure of Subcutaneous Tissue After Cesarean Section in Diabetic Patients

Surgical site infection: including the following manifestations: Collection; inflammatory signs (pain, tenderness, edema, redness); dehiscence (wound separation) or positive culture.

wound seroma, superficial wound breakdown (defined as skin and/or subcutaneous dehiscence with intact fascial layer).

Postoperative pain (judged after 24 hours, through visual analogue scale (VAS); with 0 meaning no pain, and 10 meaning the worst pain).

postoperative fever (defined as temperature more than thirty eight degree, 48 hours postoperative.

Is cosmetic wound outcome for one month postoperative good or no?

The aim of this study is to determine the surgical site infection rate and the patient's satisfaction for closure of subcutaneous tissue versus non closure of subcutaneous tissue after cesarean delivery in women with diabetes mellitus.

antiseptic/nekrosis

Early Healing of Oral Soft Tissues: a Clinical and Biomolecular Analysis. Part II

Closure Versus Non Closure of Subcutaneous Tissue After Cesarean Section in Diabetic Patients

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