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Real-time polymerase chain reaction-based approach for quantification of the pat gene in the T25 Zea mays event.

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In Europe, a growing interest for reliable techniques for the quantification of genetically modified component(s) of food matrixes is arising from the need to comply with the European legislative framework on novel food products. Real-time polymerase chain reaction (PCR) is currently the most

Differential effects of dietary Oenothera, Zizyphus mistol, and corn oils, and essential fatty acid deficiency on the progression of a murine mammary gland adenocarcinoma.

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The modulating effect of dietary enrichment in mistol seed oil (MO) containing 25% of alpha-linolenic acid (ALA), evening primrose oil (EPO) enriched in gamma-linolenic acid (GLA) and corn oil (CO) as sources of omega-6 and omega-9 fatty acids on the growth parameters of one transplantable mammary

Behavioral changes following PCB 153 exposure in the spontaneously hypertensive rat - an animal model of Attention-Deficit/Hyperactivity Disorder.

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BACKGROUND Attention-Deficit/Hyperactivity Disorder (ADHD) is a behavioral disorder affecting 3-5% of children. Although ADHD is highly heritable, environmental factors like exposure during early development to various toxic substances like polychlorinated biphenyls (PCBs) may contribute to the

Energy and fructose from beverages sweetened with sugar or high-fructose corn syrup pose a health risk for some people.

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Sugar intake in the United States has increased by >40 fold since the American Revolution. The health concerns that have been raised about the amounts of sugar that are in the current diet, primarily as beverages, are the subject of this review. Just less than 50% of the added sugars (sugar and

Corn stalk residue may add antibiotic-resistant bacteria to manure composting piles

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Manure is commonly used as a fertilizer or soil conditioner; however, land application of untreated manure may introduce pathogens and antibiotic-resistant bacteria (ARB) into the soil, with harmful implications for public health. Composting is a manure management practice wherein a carbon-rich

Unmasking differential effects of rosiglitazone and pioglitazone in the combination treatment with n-3 fatty acids in mice fed a high-fat diet.

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Combining pharmacological treatments and life style interventions is necessary for effective therapy of major diseases associated with obesity, which are clustered in the metabolic syndrome. Acting via multiple mechanisms, combination treatments may reduce dose requirements and, therefore, lower the

Isolation of infection-specific sequence tags expressed during early stages of maize anthracnose disease development.

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Summary Thirteen infection-specifically expressed sequence tags (ESTs) corresponding to genes activated during early stages of the interaction between the hemibiotrophic fungus and causal agent of anthracnose Colletotrichum graminicola, and its host, Zea mays, have been cloned. Six fungal ESTs

Evaluation of biochemical and redox parameters in rats fed with corn grown in soil amended with urban sewage sludge.

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The increased production of urban sewage sludge requires alternative methods for final disposal. A very promising choice is the use of sewage sludge as a fertilizer in agriculture, since it is rich in organic matter, macro and micronutrients. However, urban sewage sludge may contain toxic substances

Comparative analysis of thylakoid protein complexes in the mesophyll and bundle sheath cells from C3 , C4 and C3 -C4 Paniceae grasses.

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To better understand the coordination between dark and light reactions during the transition from C₃ to C₄ photosynthesis, we optimized a method for separating thylakoids from mesophyll (MC) and bundle sheath cells (BSCs) across different plant species. We grew six Paniceae

Behavioural hyperactivity in rats following postnatal exposure to sub-toxic doses of polychlorinated biphenyl congeners 153 and 126.

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Rats were exposed through mother's milk either to the di-ortho-substituted polychlorinated biphenyl (PCB) congener 2,2',4,4',5,5'-CB (IUPAC no. 153) or to the non-ortho-substituted PCB congener 3,3',4,4',5-CB (IUPAC no. 126). The different groups of mothers were dosed via gavage with corn oil

Re-evaluation of the 2-year chloroform drinking water carcinogenicity bioassay in Osborne-Mendel rats supports chronic renal tubule injury as the mode of action underlying the renal tumor response.

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Chloroform, generally regarded as a non-genotoxic compound, is associated with the induction of liver and/or kidney tumors in laboratory mice and rats. In particular, chloroform produced renal tubule tumors in low incidence in male Osborne-Mendel rats when administered by corn-oil gavage or in the

Probing Enzymatic Structure and Function in the Dihydroxylating Sesquiterpene Synthase ZmEDS

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Terpene synthases (TPSs) play a vital role in forming the complex hydrocarbon backbones that underlie terpenoid diversity. Notably, some TPSs can add water prior to terminating the catalyzed reaction, leading to hydroxyl groups, which are critical for biological activity. A particularly intriguing

Neonicotinoid insecticides alter induced defenses and increase susceptibility to spider mites in distantly related crop plants.

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BACKGROUND Chemical suppression of arthropod herbivores is the most common approach to plant protection. Insecticides, however, can cause unintended, adverse consequences for non-target organisms. Previous studies focused on the effects of pesticides on target and non-target pests, predatory

Timing and biosynthetic potential for carotenoid accumulation in genetically diverse germplasm of maize.

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Enhancement of the carotenoid biosynthetic pathway in food crops benefits human health and adds commercial value of natural food colorants. However, predictable metabolic engineering or breeding is limited by the incomplete understanding of endogenous pathway regulation, including rate-controlling

Dietary conjugated linoleic acid alters hepatic lipid content and fatty acid composition in broiler chickens.

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We examined the effect of dietary conjugated linoleic acid (CLA) on growth performance and liver composition in broiler chickens. Day-old male broiler chicks were assigned to receive a diet supplemented with corn oil (5%; n = 48) or CLA (5%; n = 48) for 21 d. Broilers fed CLA weighed less and grew

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