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Purification by oligo(dT)-cellulose of viral-specific RNA from sarcoma virus-transformed mammalian nonproducer cells.
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Viral-specific RNA has been purified by oligo(dT)-cellulose chromatography from sarcoma virus-transformed nonproducer cells. This RNA comprises approximately 3% of the purified RNA, as judged by RNA-DNA hybridization.
Peri-renal sarcoma induced by cellulose wrapping.
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[Production of subcutaneous sarcomas in rats by cellulose membranes of known porosity].
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Tumorigenicity of cellulose fibers injected into the rat peritoneal cavity.
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Cellulose fibers, along with many other organic fibers, are durable. Therefore, if inhaled, they have the potential to persist within the lung, and may then cause disease. Here we report the effects of injecting high-purity cellulose fibers into the abdominal cavity of rats. A respirable fraction of
Purification and characterization of an AP endonuclease/DNA 3' repair diesterase from mouse ascites sarcoma cells.
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Purification and characterization of a DNA repair enzyme having 5' apurinic/apyrimidinic (AP) endonuclease activity are reported. The enzyme extracted from mouse ascites sarcoma (SR-C3H/He) cells with 0.2 M potassium phosphate buffer (pH 7.5) was purified by successive chromatographies on
Protein kinase and its regulatory effect on reverse transcriptase activity of Rous sarcoma virus.
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We have studied the effect of protein phosphokinase (EC 2.7.1.37; ATP:protein phosphotransferase) and phosphoprotein phosphatase (EC 3.1.3.16; phosphoprotein phosphohydrolase) on reverse transcriptase (RNA-dependent DNA nucleotidyltransferase) activity of Rous sarcoma virus. Protein kinase from Rous
Activation of the 1,25-dihydroxyvitamin D3 receptor in cultured rat osteogenic sarcoma cells.
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Activation of the 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] receptor-hormone complex was studied in-vitro using cytosolic preparations of rat osteogenic sarcoma cell ROS 17/2-8 and a DNA-cellulose assay. We found that salt was required for extraction of the unoccupied receptor indicating its possible
The homopolyadenylate and adjacent nucleotides at the 3'-terminus of 30-40s RNA subunits in the genome of murine sarcoma-leukemia virus.
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Adenosine is the major 3'OH-terminal nucleoside of the 60-70S RNA genome of the murine sarcoma-leukemia virus, its 30-40S RNA subunits, and the poly(A) segments derived by RNase treatment of both RNA species, as determined by periodate oxidation-[(3)H]-borohydride reduction. The binding 30-40S RNA
Separation of cytidine diphosphate reductase from rat Yoshida ascites sarcoma.
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CDP reductase was separated from the cytosol of rat Yoshida ascites sarcoma. The precipitate, which resulted from the acidification of the cytosol by acetic acid at pH 5.2, catalyzed specifically the reduction of CDP, whereas the concurrently resulted supernatant catalyzed those of UDP, ADP and GDP.
Reverse transformation of Harvey murine sarcoma virus-transformed NIH/3T3 cells by site-selective cyclic AMP analogs.
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Eighteen site-selective cAMP analogs modified at either the C-8 position or the C-6 position were tested for their growth regulatory effects on the Harvey murine sarcoma virus-transformed NIH/3T3 clone 13-3B-4 cells grown in a serum-free defined medium. All 18 analogs, when tested individually,
1,25-Dihydroxyvitamin D3 receptor-like macromolecule in rat osteogenic sarcoma cell lines.
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The hormonal metabolite of vitamin D3, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), exerts its biological effects by initially binding to a cytosolic receptor protein. Such a protein has been demonstrated in the target organs of vitamin D3 including bone. Although the role of 1,25(OH)2D3 on the skeleton
Distinguishable transformation-defective phenotypes among temperature-sensitive mutants of Rous sarcoma virus.
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Eight transformation-defective, temperature-sensitive (ts) mutants of the Prague strain of Rous sarcoma virus, subgroup A, have been isolated after mutagenesis with 5-bromodeoxyuridine followed by selection on the basis of focus tests. Five of these mutants, ts GI201, GI202, GI203, GI204, and GI205,
Plasminogen activator released as inactive proenzyme from murine cells transformed by sarcoma virus.
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We have previously reported the purification of a plasminogen-activating serine protease with an approximate Mr of 48 000 from sarcoma-virus-transformed murine cells. We now report that under serum-free conditions the enzyme is released from the cells in an inactive form. After affinity
Purification of a tyrosine-specific protein kinase from Rous sarcoma virus-induced rat tumor.
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We have identified a tyrosine kinase activity present in tumors which were raised in rats by subcutaneous injection of Rous sarcoma virus-transformed rat cells (SR-NRK). This kinase phosphorylates tyrosine on the heavy chain of IgG from tumor-bearing rabbit (TBR) sera specific for the src gene
DNA strand breaks and repair synthesis in Yoshida sarcoma: cells with differential sensitivities to bi-functional alkylating agents and UV light.
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The induction of DNA-strand breaks and repair synthesis has been examined in cultured Yoshida sarcoma cell lines sensitive (YS) and resistant (YR) to methylene dimethanesulphonate (MDMS). Using an alkaline DNA unwinding-hydroxylapatite technique, we were able to detect breaks in DNA immediately
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