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Characterization of feruloyl esterases in maize pollen.
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Ferulic acid esterases have been identified and partially purified from maize pollen. Results suggest that maize pollen FAEs may play an important role in pollen fertilization. A critical step in maize (Zea mays) seed production involves fertilization of the ovule by pollen, a process that relies on
Chlorpyrifos: assessment of potential for delayed neurotoxicity by repeated dosing in adult hens with monitoring of brain acetylcholinesterase, brain and lymphocyte neurotoxic esterase, and plasma butyrylcholinesterase activities.
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Previous work has shown that acute exposures to chlorpyrifos (CPS; diethyl 3,5,6-trichloro-2-pyridyl phosphorothionate) cannot produce > 70% inhibition of brain neurotoxic esterase (NTE) and cause organophosphorus compound-induced delayed neurotoxicity (OPIDN) unless the dose is well in excess of
Plasma esterase-1 (ES-1) activity in rats is influenced by the amount and type of dietary fat, and butyryl cholinesterase activity by the type of dietary fat.
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In previous work, we studied, under conditions of ad libitum food consumption, the effect of amount and type of dietary fat on plasma esterase-1 (ES-1) and butyryl cholinesterase activity in rats. This was done by the isoenergetic replacement of dietary fat by carbohydrates or by another fat source.
Detoxication of paraoxon by rat liver homogenate and serum carboxylesterases and A-esterases.
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Paraoxon, the active metabolite of parathion, can be detoxified through a noncatalytic pathway by carboxylesterases and a catalytic pathway by calcium-dependent A-esterases, producing p-nitrophenol as a common metabolite. The detoxication patterns of carboxylesterases and A-esterases were
Protective role of melatonin on PCB (Aroclor 1,254) induced oxidative stress and changes in acetylcholine esterase and membrane bound ATPases in cerebellum, cerebral cortex and hippocampus of adult rat brain.
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Polychlorinated biphenyls (PCBs) are one of the environmental toxicants and neurotoxic compounds which induce the production of free radicals leading to oxidative stress. Membrane proteins that control ion gradients across organellar and plasma membranes appear to be particularly susceptible to
Influence of dietary fats on butyrylcholinesterase and esterase-1 (ES-1) activity in plasma of rats.
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We studied the effects of dietary fats, especially fish oil, on the activities of esterase-1 (ES-1) and butyrylcholinesterase in the plasma of rats. The identification of nutritional determinants of these enzymes could provide clues as to their physiological function. Fish oil, when compared with
Plasma esterase-1 (ES-1) activity is increased in rats fed high-fat diets.
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The question addressed is whether the amount and type of dietary fat affects esterases in plasma. Rats were fed semipurified diets containing 2.0 to 19.4% (w/w) of fat in the form of coconut fat or corn oil. Fat was added to the diets at the expense of isocaloric amounts of carbohydrates. Plasma
Influence of hypocholesterolemic drugs on aortic cholesterol esterase in rabbits.
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We have studied the influence of three hypocholesterolemic drugs (Fenofibrate, Pirinixil and Probucol) on aortic cholesterol esterase (E.C.3.1.1.13) activity in cholesterol-fed rabbits. After three weeks, cholesterol-fed controls exhibited a 28% increase in cholesteryl ester synthetase activity (S)
Characterization of anther differentiation in cytoplasmic male sterile maize using a specific isozyme system (esterase).
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During anther development, characterized in maize plants with N cytoplasm, certain esterase isozymes in non-microspore cells decrease in amount with anther age and new isozymes appear in the developing microspores. In anthers from male sterile plants with cms T or cms C cytoplasm, neither of these
Specific effect of the amount of dietary fat on esterase-1 (ES-1) activity of rat plasma.
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The question addressed is whether the amount of dietary fat rather than that of carbohydrates or protein affects esterase-1 (ES-1) activity in plasma of rats. For this purpose, the effects on plasma ES-1 activity of replacement of dietary fat, by isocaloric amounts of either carbohydrates or protein
Histochemical studies on reserve substances and enzymes in female gametophyte of Zea mays.
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Cytochemical changes during the early development of maize caryopsis are reported. Changes in the localization of different reserve substances (e.g. polysaccharides, proteins, nucleic acids and lipids) and enzymes (acid phosphatase, esterase, lipase, phosphorylase, succinate dehydrogenase,
[3H]Indole-3-acetyl-myo-inositol hydrolysis by extracts of Zea mays L. vegetative tissue.
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[3H]Indole-3-acetyl-myo-inositol was hydrolyzed by buffered extracts of acetone powders prepared from 4 day shoots of dark grown Zea mays L. seedlings. The hydrolytic activity was proportional to the amount of extract added and was linear for up to 6 hours at 37 degrees C. Boiled or alcohol
High-fat diets and fecal level of reductase and colon mucosal level of ornithine decarboxylase, beta-glucuronidase, 5'-nucleotidase, ATPase, and esterase in mice.
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In one experiment Swiss mice were maintained on a 16 or 23% fat diet (laboratory chow with added fat, principally corn oil) or on laboratory chow alone (5.5% fat). In another experiment C57BL/1 mice were given a 23% fat diet (as above) or a low-fat diet (67% laboratory chow, 1.9% corn oil, and 31%
Heterologous production and characterization of a chlorogenic acid esterase from Ustilago maydis with a potential use in baking.
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Ustilago maydis, an edible mushroom growing on maize (Zea mays), is consumed as the food delicacy huitlacoche in Mexico. A chlorogenic acid esterase from this basidiomycete was expressed in good yields cultivating the heterologous host Pichia pastoris on the 5L bioreactor scale (reUmChlE;
Effects of Two Protein Hydrolysates Obtained From Chickpea (Cicer arietinum L.) and Spirulina platensis on Zea mays (L.) Plants.
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Two liquid protein hydrolysates obtained from chickpea (Cicer arietinum L.) (CA) and Spirulina platensis (SP) were analyzed via FT-IR and SERS spectroscopy. Their hormone-like activities and contents in indole-3-acetic acid (IAA), isopentenyladenosine (IPA), nitrogen (N), carbon (C),
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