This cohort will consist of patients as described below:
15 patients with non-small cell lung cancer (NSCLC)
10 patients with hepatocellular cancer:
10 patients with colorectal cancer
10 patients with breast cancer
10 patients with prostate cancer
10 patients with glioblastoma

Oncology

During the inclusion visit (for the 5 cancer sites): Stool collection (1 tube RNA later + 1 tube DMQO-EDTA) +Blood sample (1 tube SST + 1 tube EDTA)
1 month after treatment (only for non-small cell lung cancer): Stool collection (1 tube RNA later + 1 tube DMQO-EDTA) + Blood sample (1 tube SST + 1 tube EDTA)

Collection of stool and serum samples

Centre Georges-François Leclerc

Hopital Universitaire Paul Brousse

Characteristics of the Intestinal Microbiota in Patients With Cancer

Characteristics of the Intestinal Microbiota in Patients With Cancer (Catalogue-Onco)

Bio-computer and bio-statistical analyzes will be carried out in order to constitute the gene catalog.

Centre Georges Francois Leclerc

Enterome

This cohort will consist of 55 patients as described below:
non-small cell lung cancer (NSCLC)
hepatocellular cancer:
colorectal cancer
breast cancer
prostate cancer
glioblastoma

Use Flow cytometry (FCM) and RT-PCR to test peripheral blood mononuclear cells(PBMCs) from healthy volunteer.

Use Flow cytometry (FCM) and RT-PCR to test CTCs from patients who received cryosurgery only, 1 day before and 2 days after the cryosurgery.

Use Flow cytometry (FCM) and RT-PCR to test CTCs from patients who received DC-CIK treatment only, 1 day before and 2 days after the DC-CIK treatment.

Use Flow cytometry (FCM) and RT-PCR to test CTCs from patients received cryosurgery and DC-CIK treatment both, 1 day before and 2 days after the cryosurgery with DC-CIK treatment.

Neoplastic Cells, Circulating

Use FCM to test PBMCs/CTCs from volunteers/patients.

Flow cytometry (FCM)

Use RT-PCR to test PBMCs/CTCs from volunteers/patients.

ethylene/kanker payudara

Characteristics of the Intestinal Microbiota in Patients With Cancer

The Detection of Circulating Tumor Cells (CTCs) in Patients With Breast Cancer Undergoing Cryosurgery Combined With DC-CIK Treatment

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