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Novel hydrophilic camptothecin derivatives conjugated to branched glycerol trimer suppress tumor growth without causing diarrhea in murine xenograft models of human lung cancer.
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Camptothecin possesses broad antitumor spectra on various cancers. In spite of its marked tumor suppressing potency, camptothecin is too hydrophobic to be solved in water, and therefore it is not currently in clinical use. CPT-11 (irinotecan) is one of the hydrophilic analogs of camptothecin and
Purification of an outer capsid glycoprotein of neonatal calf diarrhea virus and preparation of its antisera.
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An outer capsid glycoprotein, VP 7, was purified from a bovine rotavirus, neonatal calf diarrhea virus, by isoelectric focusing in glycerol gradients after disruption of the outer capsid. Its isoelectric point was found to be about 4.5. Guinea pigs were immunized with this VP 7 preparation. The
Travelers' diarrhea in West Africa and Mexico: fecal transport systems and liquid bismuth subsalicylate for self-therapy.
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The goals of this study were threefold: to compare the etiology of travelers' diarrhea in West Africa and Mexico, to evaluate two fecal transport systems for the recovery of enteropathogens, and to verify the efficacy of liquid bismuth subsalicylate (BSS) in different locations and under different
[Diagnosis of entero-invasive Escherichia coli associated with diarrhea].
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Sixteen Escherichia coli strains isolated from diarrhea patients, characterized as enteroinvasive (EIEC) by the keratoconjunctivitis test (Sereny test), and 15 non-EIEC strains, were studied. The aim of this study was to establish a possible correlation between the Sereny test and several other
The effect of high concentrations of cryoprotectants on the passage of bovine viral diarrhea virus through the zona pellucida of in vitro fertilized embryos.
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The effect of high concentrations of cryoprotectants on the passage of bovine viral diarrhea virus (BVDV) through the zona pellucida (ZP) of intact bovine embryos during the pre-freezing step of cryopreservation was investigated in a series of experiments. In vitro fertilized (IVF) embryos at the
An original simple technique for diagnosis of Microsporidia using glycerol jelly direct smear.
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Microsporidia spp. are obligate intracellular parasites which are very minute with sizes ranging from 1 to 10 μm. They have been increasingly recognized as human pathogens in AIDS and immunocompromised patients, mainly associated with life-threatening chronic diarrhea and systemic disease. For
Application of single-strand conformation polymorphism to the study of bovine viral diarrhea virus isolates.
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Single-strand conformation polymorphism (SSCP) analysis of polymerase chain reaction (PCR) products is a genetic screening technique for rapid detection of nucleotide substitutions in PCR-amplified genomic DNA or cDNA. It is based on the observation that partially formamide-denatured double-stranded
Assessment of the effect of esterified propoxylated glycerol (EPG) on the status of fat-soluble vitamins and select water-soluble nutrients following dietary administration to humans for 8 weeks.
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This double-blind, randomized, controlled study assessed the effect of esterified propoxylated glycerol (EPG) on fat-soluble vitamins and select nutrients in human subjects. For 8 weeks, 139 healthy volunteers consumed a core diet providing adequate caloric and nutrient intakes. The diet included
Safety and efficacy of glycerol phenylbutyrate for management of urea cycle disorders in patients aged 2months to 2years.
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Glycerol phenylbutyrate (GPB) is approved in the US for the management of patients 2months of age and older with urea cycle disorders (UCDs) that cannot be managed with protein restriction and/or amino acid supplementation alone. Limited data exist on the use of nitrogen conjugation agents in very
Identification of Vibrio hollisae sp. nov. from patients with diarrhea.
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The name Vibrio hollisae (synonym = Special Bacteriology group EF-13) is proposed for a new group of 16 strains that occurred in stool cultures of patients with diarrhea. V. hollisae is a small gram-negative rod, which is motile with a single polar flagellum. No lateral or peritrichous flagella were
[Evaluation of three new culture media: brilliant-glycerol-lactose novobiocin-green agar, modified iron lysin agar and Rambach agar for the isolation of enteropathogenic E. coli, Salmonella sp. in acute gastroenteritis].
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BACKGROUND
Acute gastroenteritis (AGE) is one of the most common diseases in children, particularly in those under the age of 5 years in whom a severe picture of hydroelectrolyte imbalance may be triggered accompanied, in most cases, by leukocyte response. The aim of this study was to evaluate three
Biochemical and morphological investigations of the toxicity of a Capmul preparation and a bile salt-EDTA solution in patients with bile duct stones.
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Seventeen patients with bile duct calculi were treated alternately with a preparation of glycerol octanoate and a bile salt-EDTA solution via a nasobiliary tube. Of 14 patients (group I), it was necessary to operate on four immediately after irrigation therapy, success resulted from this
Culturing and maintaining Clostridium difficile in an anaerobic environment.
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Clostridium difficile is a Gram-positive, anaerobic, sporogenic bacterium that is primarily responsible for antibiotic associated diarrhea (AAD) and is a significant nosocomial pathogen. C. difficile is notoriously difficult to isolate and cultivate and is extremely sensitive to even low levels of
Malabsorption and mucosal abnormalities of the small intestine in the acquired immunodeficiency syndrome.
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Diarrhea and weight loss may accompany the acquired immunodeficiency syndrome. We studied 30 patients with the syndrome, 20 of whom had diarrhea and weight loss and 10 of whom did not. Patients with identifiable enteric infections or small intestinal Kaposi's sarcoma were excluded. Malabsorption was
Metabolomics approach to serum biomarker for loperamide-induced constipation in SD rats.
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Loperamide has long been known as an opioid-receptor agonist useful as a drug for treatment of diarrhea resulting from gastroenteritis or inflammatory bowel disease as well as to induce constipation. To determine and characterize putative biomarkers that can predict constipation induced by
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