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laccase/tusam

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Potential roles of laccases on virulence of Heterobasidion annosum s.s.

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Laccases, multi-copper-containing proteins, can catalyze the oxidation of phenolic substrates and have diverse functions such as a virulence factor in fungi. However, limited information can be found on the role of laccases in the interaction of Heterobasidion annosum s.s. to its host plant. Due to
The white-rot basidiomycete Ceriporiopsis subvermispora produces two families of ligninolytic enzymes, namely manganese-dependent peroxidases (MnPs) and laccases, when growing in liquid cultures of defined composition. In medium containing 11 p.p.m. of Mn(II), up to seven isoenzymes of MnP and four

Characterization of two laccases of loblolly pine (Pinus taeda) expressed in tobacco BY-2 cells.

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We previously showed that eight laccase genes (Lac 1-Lac 8) are preferentially expressed in differentiating xylem and are associated with lignification in loblolly pine (Pinus taeda) [Sato et al. (2001) J Plant Res 114:147-155]. In this study we generated transgenic tobacco suspension cell cultures

Oxidase activity in lignifying xylem of a taxonomically diverse range of trees: identification of a conifer laccase.

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In a diverse taxonomic range of tree species, including representative species of ancient families of angiosperms (Magnolia x soulangiana Soul.-Bod.) and gymnosperms (Ginkgo biloba L.), oxidase activity was associated with cell walls of developing xylem and was enriched in extracts of cell

Electrophoretic analysis of coniferyl alcohol oxidase and related laccases.

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Gradient gel electrophoretic methods enabled a distinction to be made between coniferyl alcohol oxidase (CAO) of lignifying cell walls and a pI approximately 9 pine "laccase" recently implicated in lignification (Science 1993 260, 672). Following treatment of a partially purified protein mixture
The capacity of laccase from Myceliophthora thermophila to promote the oxidative polymerisation of Kraft lignin (KL) was evaluated in several conditions of pH, temperature, enzyme dosage and treatment time. Moreover, different black liquors from the Kraft cooking of Eucalyptus globulus and mixture

Evaluation of laccase production by Ganoderma lucidum in submerged and solid-state fermentation using different inducers.

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Laccases are multicopper oxidases with high potential for industrial applications. Several basidiomycete fungi are natural producers of this enzyme; however, the optimization of production and selection of inducers for increased productivity coupled with low costs is necessary. Lignocellulosic

A laccase associated with lignification in loblolly pine xylem.

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Peroxidase has been thought to be the only enzyme that oxidizes monolignol precursors to initiate lignin formation in plants. A laccase was purified from cell walls of differentiating xylem of loblolly pine and shown to coincide in time and place with lignin formation and to oxidize monolignols to

Generation and analysis of expressed sequence tags from six developing xylem libraries in Pinus radiata D. Don.

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BACKGROUND Wood is a major renewable natural resource for the timber, fibre and bioenergy industry. Pinus radiata D. Don is the most important commercial plantation tree species in Australia and several other countries; however, genomic resources for this species are very limited in public

Identification of putative candidate genes for juvenile wood density in Pinus radiata.

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Wood formation is a complex developmental process driven by the annual activity of the vascular cambium. Conifers usually produce juvenile wood at young ages followed by mature wood for the rest of their lifetime. Juvenile wood exhibits poorer wood quality (i.e., lower density) compared with mature

The role of endophytic fungal individuals and communities in the decomposition of Pinus massoniana needle litter.

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The role of fungal endophytes (FEs) as "pioneer" decomposers has recently been recognized; however, the extent to which FEs contribute to litter loss is less well understood. The genetic and enzymatic bases of FE-mediated decomposition have also rarely been addressed. The effects of populations and
Solid-state fermentation (SSF) with the medicinal higher Basidiomycete Ganoderma lucidum was studied as a strategy to use pine (Pinus radiata D. Don) and poplar (Populus nigra L.) wood chips and sawdust. Fruiting bodies were produced and the value of the biotransformed substrate was assessed. The

Rhizosphere dynamics during phytoremediation of olive mill wastewater.

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The potential of phytoremediation as a treatment option for olive mill wastewater (OMW) was tested on five perennial tree species. Cupressus sempervirens and Quercus ilex proved tolerant to six-month OMW treatment followed by six-month water irrigation, whereas Salix sp. and Laurus nobilis and,
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