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12 hasil
Cyst germination proteins of the potato pathogen Phytophthora infestans share homology with human mucins.
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We have cloned genes of Phytophthora infestans, the causal agent of potato late blight, that are activated shortly before the onset of invasion of the host tissue. The three genes isolated appear to be arranged in a genomic cluster and belong to a small polymorphic gene family. A conspicuous feature
Influence of dietary protein level and origin on the flow of mucin along the small intestine of the preruminant calf.
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The objective of this study was to investigate the effect of the dietary crude protein (CP) content and origin on the flow of mucin protein along the small intestine of the preruminant calf. Diets contained 1, 10, 20 and 28% of CP supplied by skim milk powder (SMP) in experiment 1. Diets differed by
Effect of dietary graded resistant potato starch levels on growth performance, plasma cytokines concentration, and intestinal health in meat ducks.
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The objective of the present study was to investigate the effect of dietary graded raw potato starch (RPS) levels on growth performance, plasma cytokines concentration, ileal barrier function, and cecal short-chain fatty acids (SCFA) concentration in meat ducks from 1 to 35 D of age. This study
Gastric MUC5AC and MUC6 are large oligomeric mucins that differ in size, glycosylation and tissue distribution.
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Gastric MUC5AC and MUC6 mucins were studied using polyclonal antibodies. Immunohistochemistry showed MUC5AC to originate from the surface epithelium, whereas MUC6 was produced by the glands. Mucins from the surface epithelium or glands of corpus and antrum were purified using CsCl/4M guanidinium
Effect of using multiple culture media for the diagnosis of noninvasive fungal sinusitis.
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BACKGROUND
This study was designed to assess the relative efficiency of three different culture media for isolating fungi in patients suspected of having noninvasive fungal sinusitis.
METHODS
A prospective study was performed of 209 operative samples of sinus "fungal-like" mucin from 134 patients on
Molecular identification of Schizophyllum commune as a cause of allergic fungal sinusitis.
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Schizophyllum commune, a basidiomycetous fungus, rarely causes disease in humans. We report a rare case of allergic fungal sinusitis caused by S. commune in a 14-yr-old girl. The patient presented with nasal obstruction and a purulent nasal discharge. Materials obtained during endoscopic surgery of
Poly-N-acetyllactosamine-specific tomato lectin interacts with gastric parietal cells. Identification of a tomato-lectin binding 60-90 X 10(3) Mr membrane glycoprotein of tubulovesicles.
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The cytoplasmic tubulovesicular and canalicular membranes of gastric parietal cells are intimately involved in hydrochloric acid secretion. To characterise the glycoproteins of these membranes, we examined a panel of lectins for reactivity with parietal cells in paraffin sections of rat, dog and pig
Inhibitory effects of sialic acid- or N-acetylglucosamine-specific lectins on histamine release induced by compound 48/80, bradykinin and a polyethylenimine in rat peritoneal mast cells.
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The effects of seven lectins with various sugar-specificities on histamine release from rat peritoneal mast cells induced by non-immunologic stimuli were studied. The non-immunologic stimuli used were three basic secretagogues, compound 48/80, bradykinin and PEI6 (polyethylenimine with a molecular
Long-term intake of resistant starch improves colonic mucosal integrity and reduces gut apoptosis and blood immune cells.
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OBJECTIVE
The potential effect of a long-term intake of resistant starch on colonic fermentation and on gut morphologic and immunologic indices of interest in bowel conditions in humans was studied in pigs.
METHODS
Sixteen growing pigs were meal fed for 14 wk on a diet containing a large amount of
Development of a sensitive and reliable ELISA for quantification of wheat germ agglutinin.
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Among others, lectin-mediated drug delivery is currently discussed as a promising strategy towards improved bioavailability of biotech drugs. For quantitative determination of the lectin from wheat germ (WGA), a sandwich ELISA relying on capture of the lectin by pig gastric mucin coated wells and
Purification, characterization and biological significance of mannose binding lectin from Dioscorea bulbifera bulbils.
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Dioscorea bulbifera or air potato has been used as a folk remedy to treat cancer. A mannose binding lectin from bulbils of D. bulbifera was purified in a single step by affinity chromatography on mucin coupled Sepharose 4B column, determined by its fine sugar specificity by glycan array analysis and
Dietary administration of resistant starch improved cecal barrier function by enhancing intestinal morphology and modulating microbiota composition in meat duck.
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Resistant starch (RS) was recently approved to exert a powerful influence on gut health, but the effect of RS on the cecal barrier function in meat ducks has not been well defined. Thus, the effect of raw potato starch (RPS), a widely adopted RS material, on microbial composition and barrier
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