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Localization of lectin-binding sites and sugar-binding proteins in tachyzoites of Toxoplasma gondii.
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Lectins and neoglycoproteins labeled with colloidal gold particles were used for the ultrastructural localization of carbohydrate residues and sugar-binding sites, respectively, in thin sections of tachyzoites of Toxoplasma gondii embedded in the Lowicryl K4M resin. Incubation of the sections in the
Lectin binding to extracellularly melanized microfilariae of Brugia malayi from the hemocoel of Anopheles quadrimaculatus.
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Binding patterns of fluorescein isothiocyanate (FITC)- and gold-conjugated lectins to extracellularly melanized sheathed and exsheathed microfilariae of subperiodic Brugia malayi, isolated from and in situ in the abdominal hemocoel of Anopheles quadrimaculatus 72-hr postinfection, were examined.
Surface carbohydrate changes on Onchocerca lienalis larvae as they develop from microfilariae to the infective third-stage in Simulium ornatum.
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Use was made of seven FITC labelled lectins as tools to investigate the surface of Onchocerca lienalis larvae as they develop through to the infective third-stage in a natural vector, Simulium ornatum. The lectins were derived from Canavalia ensiformis (Con A), Lens culinaris (lentil), Triticum
Immunohistochemical investigations on the pyloric glands of the ruin lizard (Podarcis sicula campestris de Betta).
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Mucous cells and enteroendocrine cells of the pyloric region of the ruin lizard (Podarcis sicula campestris De Betta) have been examined by lectin histochemical and immunohistochemical methods. Binding to five plant lectins (Canavalia ensiformis, Con A; Triticum vulgare, wheat germ, WGL; Lotus
Distribution of lectin binding in the testes of the musk shrew, Suncus murinus.
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Distribution of lectin binding in the testis of the musk shrew, Suncus murinus, was investigated by light and transmission electron microscopy. Not only spermatogenic cells but also Sertoli cells bound some lectins. Canavalia ensiformis agglutinin (Con A) and wheat germ agglutinin (WGA, Triticum
Synthesis, micellization and lectin binding of new glycosurfactants.
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Here we report the preparation and physico-chemical characterization of carbohydrate-decorated micelles and their interaction with lectins. A library of biosourced amphiphiles was prepared by copper-catalyzed azide-alkyne cycloaddition (CuAAC) between alkynyl sugars (lactose, N-acetyl-D-glucosamine)
Expression pattern of glycoconjugates in the Bidderian and ovarian follicles of the Brazilian toad Bufo ictericus analyzed by lectin histochemistry.
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The Bidder's organ and ovary of the Brazilian toad Bufo ictericus were studied by light microscopy, using hematoxylin-eosin (HE) and periodic acid Schiff (PAS) staining. The expression and distribution of carbohydrate moieties was analyzed by lectin histochemistry, using 8 lectins with different
Lectin-dependent attachment of Actinomyces naeslundii to receptors on epithelial cells.
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The adherence of Actinomyces naeslundii WVU45 to monolayer cultures of human epithelial cell lines was mediated by the lactose-sensitive fimbriae (type 2) of strain WVU45. The attachment of Actinomyces viscosus T14V, which has both types 1 and 2 fimbriae, was approximately half that of A.
Flow cytometric analyses of lectin binding to Pneumocystis carinii surface carbohydrates.
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Pneumocystis carinii obtained from infected rat lung homogenates was incubated with fluorescein isothiocyanate-conjugated lectins, counterstained with the nuclear stain, propidium iodide (PI), and analyzed by dual parameter histograms for lectin-associated green and PI-associated red fluorescence
Characterization of glycans in the developmental stages of Myxobolus cerebralis (Myxozoa), the causative agent of whirling disease.
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Glycans and sugar-binding molecules (lectins) form an interactive recognition system, which may enable parasitic organisms to adhere to host cells and migrate into target tissues. The aim of the present study was to analyse surface-associated glycans in the developmental stages of Myxobolus
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