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The effect of hypoxia and ouabain on the muscle resting potential of Periplaneta americana L.
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We have studied the effect of hypoxia and ouabain on the muscle resting potential (RP) of the cockroach Periplaneta americana L. The experiments were done in insects reared either in normoxic or hypoxic conditions and treated, or not treated, with ouabain. Hypoxic conditions decreased the RP by
Effect of hypoxia on ouabain inhibition of sodium pump in newborn rabbit myocardium.
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The effects of hypoxia and reoxygenation on the inotropic effect of ouabain and sodium pump were studied in the isolated, arterially perfused newborn rabbit heart. Myocardial 86Rb+ active uptake was used as a marker of the sodium pump activity. During reoxygenation after mild hypoxia, 86Rb+ active
Deleterious effect of ouabain on myocardial function during hypoxia.
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The effect of cardiac glycosides on myocardial function during hypoxia is controversial. Accordingly, we studied left ventricular performance during hypoxia and reoxygenation in the presence of a mildly inotropic, nontoxic dose of ouabain using isolated, isovolumic, buffer-perfused rabbit hearts.
Comparison of hypoxia and ouabain effects on the myocardial uptake kinetics of technetium-99m hexakis 2-methoxyisobutyl isonitrile and thallium-201.
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Effects of hypoxia and ouabain on transcapillary exchange of [99mTc]hexakis (2-methoxyisobutylisonitrile) [SESTAMIBI, also known as MIBI or HEXAMIBI] and 201TI were investigated with indicator-dilution studies using isolated rabbit hearts. Peak myocardial extraction (Emax), permeability-surface area
Mechanisms of injury-induced calcium entry into peripheral nerve myelinated axons: in vitro anoxia and ouabain exposure.
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In the present investigation, electron probe X-ray microanalysis was used to characterize the effects of in vitro ouabain (2 mM) or anoxia on elemental composition (e.g. Na, K, Ca) and water content of rat peripheral (tibial) nerve myelinated axons and Schwann cells. Results showed that independent
Combined effects of ouabain and hypoxia on the transmembrane potential of of canine cardiac Purkinje fibers.
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The effects of hypoxia on the transmembrane potential of dog Purkinje cells in isolated, superfused fiber bundles pretreated with ouabain were studied. Cells stimulated electrically at 93/min were exposed to ouabain, 2.1 X 10(-7) M, until the magnitude of phase 4 depolarization increased to 7-12 mV.
Effect of in utero hypoxia on the ouabain/strophanthidin binding site of the fetal guinea pig brain cell membrane Na+,K(+)-ATPase.
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This study investigates the effect of hypoxia on the high affinity strophanthidin/ouabain binding site of brain cell membrane Na+,K(+)-ATPase in 30, 45 and 60 day (term) fetal guinea pigs. Studies were performed on 30 fetuses randomized to either normoxic or hypoxic conditions. The hypoxic fetuses
Effects of ouabain and hypoxia on the cardiac stimulation threshold in the dog (with special reference to changes in extra- and intracellular potassium concentrations).
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The influence on the cardiac stimulation threshold of changes in the myocardial intra/extracellular potassium ratio induced with ouabain and hypoxia was investigated in 9 dogs with total atrioventricular block. In spite of significant elevations of extracellular potassium and reductions of
Ouabain-induced changes of calcium and potassium in slices of hippocampus of the rat: comparison to hypoxia and effect of R 56865.
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Simple and reliable in vitro models of cerebral ischaemia are important for the identification of antiischaemic/antihypoxic compounds. Alterations of the concentrations of potassium and calcium were recorded in slices of hippocampus of the rat. The slices were subjected to hypoxia in the presence
Digoxin and ouabain induce P-glycoprotein by activating calmodulin kinase II and hypoxia-inducible factor-1alpha in human colon cancer cells.
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Digoxin and ouabain are cardioactive glycosides, which inhibit the Na+/K+-ATPase pump and in this way they increase the intracellular concentration of cytosolic calcium ([Ca2+](i)). They are also strong inducers of the P-glycoprotein (Pgp), a transmembrane transporter which extrudes several drugs,
Effects of hypoxia, 2,4-dinitrophenol and ouabain on twitch potentiation elicited following conditioning pulses in the mouse phrenic nerve-diaphragm preparation.
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Hypoxia is suggested to increase intracellular Ca2+, thereby affecting cellular functions. If this is the case, the effect would be modified by other treatments which also elevate intracellular levels of Ca2+. To test this possibility, in mouse diaphragm preparations, the effects of hypoxia were
Effects of hypoxia on the contractions and lactate release induced by high-K+, ouabain and epinephrine in guinea-pig aorta.
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1. The 60 mM K+, 152 mM K+, Na-deficient medium and oubain-induced contractions of aorta were not so affected by severe hypoxia. 2. The 60 mM K+, 152 mM K+, Na(+)-deficient medium-induced responses were greatly reduced by deprivation of external Ca2+ in normoxia. 3. As the concentration of
Effects of ouabain on the mechanical performance of heart muscle in the frog, Rana temporaria, during hypoxia and subsequent recovery at pH 6.6.
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Effects of ouabain on cerebral metabolism during anoxia.
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Effect of hypoxia, ouabain and tetrodotoxin on the parameters of optic evoked potentials in chick embryos.
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