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pinus/protease

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[Changes in proteins and proteases during artificial aging in seeds of Pinus pinea].

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Changes in the germination-rate and the biochemical differences in soluble proteins and proteases in artificially aged Pinus pinea seeds have been investigated. In the aged seeds, soluble proteins decrease during the germination process, parallel to increased levels of proteases activities in both

Cysteine proteases secreted by the pinewood nematode, Bursaphelenchus xylophilus: In silico analysis.

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The pinewood nematode, Bursaphelenchus xylophilus, is an important plant-parasitic nematode responsible for the development of the pine wilt disease and recognised as a major forest pest. Previous studies on the comparison of B. xylophilus and B. mucronatus secretomes obtained under maritime pine,

A novel Otubain-like cysteine protease gene is preferentially expressed during somatic embryogenesis in Pinus radiata.

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OTUBAINS are a recently discovered family of cysteine proteases that participate in the ubiquitin pathway. These proteins were originally described in animal systems and are involved in removing the ubiquitin chain attached to a protein destined for degradation. In a cDNA-AFLP screen designed to

Nematicidal bacteria associated to pinewood nematode produce extracellular proteases.

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Bacteria associated with the nematode Bursaphelenchus xylophilus, a pathogen of trees and the causal agent of pine wilt disease (PWD) may play a role in the disease. In order to evaluate their role (positive or negative to the tree), strains isolated from the track of nematodes from infected Pinus

Identification of Proteases and Protease Inhibitors in Allergenic and Non-Allergenic Pollen.

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Pollen is one of the most common causes of allergy worldwide, making the study of their molecular composition crucial for the advancement of allergy research. Despite substantial efforts in this field, it is not yet clear why some plant pollens strongly provoke allergies while others do not.
BACKGROUND Allergic disorders, such as seasonal rhinitis and asthma, are increasing causes of morbidity worldwide and often result from exposure to airborne pollen. Pollen allergy has a remarkable clinical impact all over Europe. In fact, epidemiological longitudinal studies confirm that pollen

On the stability of isocitrate lyase from Pinus pinea.

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Isocitrate lyase is a key catalyst of the glyoxylate cycle. A feature of the enzyme from higher plants is the high instability, that causes innumerable problems in working for characterization of the enzyme. The present communication demonstrates that the optimal conditions for the storage of

Isocitrate lyase: artifacts and multiple enzyme forms.

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Multiple enzyme forms of isocitrate lyase from various sources have been frequently reported. Protease action after cell rupture was sporadically claimed to explain the observed multiple enzyme forms. In this communication studies which are consistent with a protease action in vitro on isocitrate
Extracellular matrix (ECM) modifications occur during plant growth, development, and in response to environmental stimuli. Key modulators of ECM modification in vertebrates, the extracellular matrix metalloproteinases (MMPs), have also been described in a few plants. Here, we report the

Purification and characterization of the 7S vicilin from Korean pine (Pinus koraiensis).

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Pine nuts are economically important as a source of human food. They are also of medical importance because numerous pine nut allergy cases have been recently reported. However, little is known about the proteins in pine nuts. The purpose of this study was to purify and characterize pine nut storage

[Effects of elevated CO2 on soil hydrolase activities associated with Pinus koraiensis seedlings].

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In this study, Pinus koraiensis seedlings were grown under elevated CO2 (500 and 700 micromol x mol(-1)), and the hydrolase activities in 0 - 10 cm soil layer were determined. The results showed that compared with those under ambient condition, the activities of soil protease, urease, amylase and

Proteolytic activity in relation to seasonal cambial growth and xylogenesis in Pinus banksiana.

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Proteolytic activity in the cambial zone and developing xylem of Pinus banksiana Lamb. was investigated over an annual cycle of growth and dormancy. Highest proteolytic activity was associated with the most active period of primary-wall radial expansion of cambial derivatives, in early spring,

Inhibitory effects of Pinus massoniana bark extract on hepatitis C virus in vitro.

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BACKGROUND Given that Pinus massoniana Lamb (Pinaceae) bark extract (PMBE) is a safe and non-toxic flavonoid found abundantly in nature, it was considered a promising novel candidate agent in the treatment of virus infection. OBJECTIVE Experiments were conducted to assay the antiviral character of

PpRT1: the first complete gypsy-like retrotransposon isolated in Pinus pinaster.

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We have isolated and characterized a complete retrotransposon sequence, named PpRT1, from the genome of Pinus pinaster. PpRT1 is 5,966 bp long and is closely related to IFG7 gypsy retrotransposon from Pinus radiata. The long terminal repeats (LTRs) have 333 bp each and show a 5.4% sequence

Review on Sustained Relief of Osteoarthritis Symptoms with a Proprietary Extract from Pine Bark, Pycnogenol.

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This review summarizes the effects of the standardized proprietary bark extract of the French maritime pine (Pycnogenol®) in mild osteoarthritis (OA), stage 1 and 2. The extract exerts antioxidative, anti-inflammatory, and chondroprotective effects in vitro and in vivo. Its phenolic acids as well as
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