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Separation of two constituents from purple sweet potato by combination of silica gel column and high-speed counter-current chromatography.
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It is known that the choice of solvent system for high speed counter-current chromatography separation is of utmost importance. In this study, a simple and rapid thin layer chromatograph coupling with fluorometric (TLC-F) method has been used to determine the partition coefficient of target
Novel strategy for synthesis of magnetic dummy molecularly imprinted nanoparticles based on functionalized silica as an efficient sorbent for the determination of acrylamide in potato chips: Optimization by experimental design methodology.
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This study discusses a novel and simple method for the preparation of magnetic dummy molecularly imprinted nanoparticles (MDMINPs). Firstly, Fe3O4 magnetic nanoparticles (MNPs) were synthesized as a magnetic component. Subsequently, MDMINPs were constructed via the sol-gel strategy using APTMS as
Ames mutagenicity tests of products from a heated potato-starch system.
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A charred sample was prepared from potato starch heated with ammonium carbonate at 600 degrees C in a flask under a nitrogen stream. The water produced was collected and extracted with methylene chloride. The basic fraction obtained from the extract exhibited strong mutagenicity in Ames assays using
Synthesis of the aggregation pheromone of the Colorado potato beetle from its degradation product.
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Incubation of the Colorado potato beetle aggregation pheromone, (S)-1,3-dihydroxy-3,7-dimethyl-6-octen-2-one, with antennal or leg extracts from this beetle gave 6-methyl-5-hepten-2-one as the major product. This ketone was used as a substrate in a stereoselective synthesis of the pheromone. It was
Study on alpha-amylase hydrolysis of potato amylopectin by a quartz crystal microbalance.
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Potato amylopectin with phosphate groups was immobilized on a quartz crystal microbalance with dissipation monitoring (QCMD) using the attractive interaction between opposite charges, and enzymatic starch hydrolysis was monitored directly. Poly( L-lysine) (PLL) proved to be an appropriate cationic
The hatching factor of the potato-root eelworm.
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1. The hatching factor of the potato-root eelworm was concentrated from potato-root leachings by adsorption on charcoal. The crude material, extracted from charcoal with acetone, was purified by partition between ethyl acetate and m-potassium dihydrogen phosphate, and ethyl acetate and a solution of
High-performance thin-layer chromatographic determination of potato glycoalkaloids.
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A high-performance thin-layer chromatographic (HPTLC) method for the determination of the two main potato glycoalkaloids, alpha-chaconine and alpha-solanine, in different parts of the potato plant, is described. Samples were extracted with diluted aqueous acetic acid containing the ion-pairing
[Fatty acid composition of glycerolipids from potato leaves].
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A method for rapid isolation of glyco- and phospholipids from potato leaves by a two-fold separation in a thin layer of silica gel is described. Using gas-liquid chromatography, the fatty acid compositions of monogalactosyldiglyceride, digalactosyldiglyceride, sulfolipid, phosphatidyl choline,
[Methodological hints on the gas chromatographic determination of kelevan and kepone in potatoes, potato foliage and soil].
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The gas chromatographic determination of Kelevan, an ethyllaevulinic acid derivative of Kepone, requires its conversion to Kepone. Thermal decomposition in the injection block of the gas chromatograph at 45 degrees C. has proved to be well suited for routine work. The sample is extracted with
Nonideal size-exclusion chromatography with zwitterions causes a pI-dependent elution of proteins from glycerol propylsilane-modified silica.
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A potato homogenate containing proteins of similar molecular weights but varying pI values was separated by nonideal size-exclusion chromatography after attempts to resolve the proteins with common size-exclusion chromatography methods failed. The method used a zwitterion buffer to amplify
Exposure to respirable crystalline silica in eastern North Carolina farm workers.
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Occupational exposure to crystalline silica has been linked to silicosis, some forms of cancer, and certain autoimmune diseases. Little information exists on exposure levels of respirable silica in the agricultural industry. This study assessed respirable silica exposure of farm workers in eastern
Sweet Potato Peels and Cancer Prevention.
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A bioassay-guided fractionation of an alcoholic extract from the peels of Ipomoea batatas Lam has been carried out. Sulforhodamine B and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays were used to evaluate the anticancer and antioxidant potential, respectively, while silica gel column chromatography
Cryopreservation of non-encapsulated embryogenic tissue of sweet potato (Ipomoea batatas).
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Embryogenic tissue of the sweet potato (Ipomoea batatas (L) LAM) genotype TIB 10 was established from in vitro axillary shoot tips on Murashige and Skoog (1962) medium supplemented with 5 μM 2,4-dichlorophenoxyacetic acid. Embryogenic aggregates of fresh mass 9.0-12 mg were subjected to a rapid
[Determination of furanoterpenoid toxins from sweet potato by thin-layer chromatography].
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Sweet potato root tissue, when infected with different pathogens, invaded by insects, or irritated by certain chemicals, turns black-brown and produces and accumulates a large amount of furanoterpenoids such as ipomeamarone, ipomeamaronol, ipomeanine and 4-ipomeanol, which are toxic to animals. The
[Study of ceratocystis fimbriata toxins determination from sweet potato by high performance thin-layer chromatography].
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The extract solution from Sweet Potato was washed with 5% Na2CO3 and followed with distilled water and dried with dehydrated sodium sulfate (Na2SO4). The ether extract was evaporated and the residue (crude sample) was dissolved in a small volume of chloroform-soluble solution and was spotted on a
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