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Novel resources of Taxol from endophytic and entomopathogenic fungi: Isolation, characterization and LC-Triple mass spectrometric quantification.
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This work presents a second-to-none method for Taxol isolation from the Endophytic fungus Cladosporium sphaerospermum (AUMC 6896) and the Entomopathogenic fungus Metarizium anisopliae (AUMC 5130). The extracts were analyzed by high performance liquid chromatography (HPLC) and Liquid chromatography
[Virions and membrane proteins of the potato X virus interact with microtubules and enables tubulin polymerization in vitro].
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A study was made of the in vitro interactions of virions and the coat protein (CP) of the potato virus X (PVX) with microtubules (MT). Both virions and CP cosedimented with taxol-stabilized MT. In the presence of PVX CP, tubulin polymerized to produce structures resistant to chilling. Electron
Screening of Taxol-producing fungi based on PCR amplification from Taxus.
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Genes coding for 10-deacetylbaccatin III-10-O-acetyl transferase and C-13 phenylpropanoid side chain-CoA acyltransferase were used as molecular markers for screening of Taxol-producing endophytic fungi. Using PCR, three out of 90 endophytic fungi, isolated from Taxus x media and Taxus yunnanensis,
Isolation and identification of an anticancer drug, taxol from Phyllosticta tabernaemontanae, a leaf spot fungus of an angiosperm, Wrightia tinctoria.
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Phyllosticta tabernaemontanae, a leaf spot fungus isolated from the diseased leaves of Wrightia tinctoria, showed the production of taxol, an anticancer drug, on modified liquid medium (M1D) and potato dextrose broth (PDB) medium in culture for the first time. The presence of taxol was confirmed by
Taxol production by an endophytic fungus, Fusarium redolens, isolated from Himalayan yew.
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Different endophytic fungi isolated from Himalayan Yew plants were tested for their ability to produce taxol. The BAPT gene (C-13 phenylpropanoid side chain-CoA acetyl transferase) involved in the taxol biosynthetic pathway was used as a molecular marker to screen taxol-producing endophytic fungi.
Isolation of taxol producing endophytic fungus Alternaria brassicicola from non-Taxus medicinal plant Terminalia arjuna.
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In the present study, an endophytic fungal strain was isolated from its non-Taxus host plant Terminalia arjuna and identified as Alternaria brassicicola based on its morphological characteristics and internal transcribed spacer sequence analysis. This fungus was grown in potato dextrose broth and
Taxol from Phyllosticta citricarpa, a leaf spot fungus of the angiosperm Citrus medica.
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Phyllosticta citricarpa, a leaf spot fungus isolated from the diseased leaves of Citrus medica, displayed the production of taxol, an anticancer drug on M1D and potato dextrose broth medium in culture for the first time. The presence of taxol was confirmed by spectroscopic and chromatographic
[Isolation and identification of a taxol-producing endophytic fungus from Podocarpus].
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OBJECTIVE
Endophytic fungi can produce beneficial active components during symbiosis with host plants. We isolated a taxol-producing endophytic fungus strain from Podocrapus.
METHODS
The anti-tumor activity of the endophytic funguswas detected by Methyl Thiazolyl Tetrazolium (MTT) method with Vero
Taxol from Tubercularia sp. strain TF5, an endophytic fungus of Taxus mairei.
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The diterpenoid taxol is an important anticancer agent used widely in the clinic. The purpose of this work was to identify a taxol-producing endophytic fungus (strain TF5) isolated from Taxus mairei and study its anticancer activities. Strain TF5 was identified as a Tubercularia sp. according to the
Enhancing taxol production in a novel endophytic fungus, Aspergillus aculeatinus Tax-6, isolated from Taxus chinensis var. mairei.
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Taxol is a curatively effective but rare anti-tumor agent extracted from Taxus (yew) barks; however, the high cost and low production of the extraction method have limited its widespread use. In this study, fungi isolated from Taxus chinensis var. mairei were tested for their ability to produce
Isolation and characterization of endophytic taxol-producing fungi from Taxus chinensis.
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This study investigated the endophytic fungi diversity of Taxus chinensis and screened the taxol-producing fungi in the host. A total of 115 endophytic fungi isolates obtained from bark segments of T. chinensis were grouped into 23 genera based on the morphological traits and sequence analysis of
An endophytic taxol-producing fungus from Taxus media, Cladosporium cladosporioides MD2.
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Fermentation processes using taxol-producing fungi other than Taxus spp. may be an alternative way to produce taxol, which is an important antitumor agent used widely in the clinic setting. In this study, a taxol-producing endophytic fungus strain MD2 was isolated from the inner bark of Taxus media.
Binding of pp170 to microtubules is regulated by phosphorylation.
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We have used a monoclonal antibody affinity column to purify from HeLa cells a protein of molecular weight 170,000 (designated pp170) which we previously identified as a nucleotide-sensitive microtubule-binding protein (Rickard, J. E., and Kreis, T. E. (1990) J. Cell Biol. 110, 1623-1633). We show
Studies on the Chemical Diversities of Secondary Metabolites Produced by Neosartorya fischeri via the OSMAC Method.
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The One Strain Many Compounds (OSMAC) method was applied to explore the chemical diversities of secondary metabolites produced by Neosartorya fischeri NRRL 181. Four pyripyropenes 1⁻4, eight steroids 5⁻11, and four prenylated indole alkaloids 12⁻15, were obtained from the fungus cultured in petri
An endophytic Basidiomycete, Grammothele lineata, isolated from Corchorus olitorius, produces paclitaxel that shows cytotoxicity.
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Grammothele lineata, an endophyte isolated in our laboratory from jute (Corchorus olitorius acc. 2015) was found to be a substantial paclitaxel producer. Taxol and its related compounds, produced by this endophyte were extracted by growing the fungus in simple nutrient media (potato dextrose broth,
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