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theobromine/dichloromethane
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The simultaneous determination of theophylline, theobromine and caffeine in plasma by high performance liquid chromatography.
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A high performance liquid chromatographic procedure for the stimultaneous micro-scale determination of theophylline, theobromine and caffeine in plasma is described. After a single dichloromethane extraction of 0.5--0.2 ml of acidified plasma, the evaporated residue is chromatographed on a
[Development of syringe-type off line pre-column and simultaneous quantitation of four xanthine derivatives (caffeine, theobromine, theophylline and paraxanthine) in serum].
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A new syringe-type minicolumn, called Extrashot-Silica (EXS-Silica), containing diatomaceous earth granules was described. The EXS-Silica differs from the conventional pretreatment column. Using the EXS-Silica we can execute the simultaneous extraction-injection to HPLC, column. Therefore, an
Determination of caffeine in coffee products by dynamic complexation with 3,4-dimethoxycinnamate and separation by CZE.
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A method based on the formation of pi-complexes with chlorogenate-like species was proposed for the determination of caffeine in regular (nondecaffeinated) and decaffeinated coffee. Both caffeate and 3,4-dimethoxycinnamate were able to transform caffeine--a neutral species in aqueous solutions--into
Simultaneous determination of methylxanthines in different types of tea by a newly developed and validated TLC method.
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The aim of this paper is to develop a new simple, fast and economical method for simultaneous quantitative determination of methylxanthine compounds based on TLC combined with image analysis. To obtain certain results, both extraction and chromatographic separation were optimized. The optimum
High-performance liquid chromatographic determination of dimethylxanthine metabolites of caffeine in human plasma.
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A normal-phase high-performance liquid chromatographic assay of caffeine and its metabolites, theophylline, theobromine and paraxanthine, in human plasma is described. The two internal standards ethyltheophylline and 1,3,7-trimethyluric acid are used simultaneously and cover the range of different
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