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zeatin riboside/jagung
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Concentration Gradients of trans-Zeatin Riboside and trans-Zeatin in the Maize Stem: Measurement by a Specific Enzyme Immunoassay.
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A sensitive, specific enzyme immunoassay (EIA) for trans-zeatin riboside (ZR) and trans-zeatin (Z) in the 0.3 to 30 picomole range has been described. The reliability of the method for measuring ZR + Z in partially purified extracts of Zea mays L. tissues was verified by highperformance liquid
Regulators of cell division in plant tissues : XVIII. Metabolism of zeatin in Zea mays seedlings.
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[(3)H] Zeatin was supplied to Zea mays L. seedlings with roots excised; the metabolites identified were adenosine-5'-phosphate, adenosine, adenine, and 7-glucosylzeatin (a minor metabolite). The principal metabolites formed from zeatin by the roots of intact Z. mays seedlings were
High-level expression and characterization of Zea mays cytokinin oxidase/dehydrogenase in Yarrowia lipolytica.
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Cytokinin oxidase/dehydrogenase (CKO/CKX) is a flavoenzyme, which irreversibly inactivates cytokinins by severing the isoprenoid side chain from the adenine/adenosine moiety. There are several genes coding for the enzyme in maize (Zea mays). A Z. mays CKO1 cDNA was cloned in the yeast Yarrowia
Quantitation of cytokinins in biological samples using antibodies against zeatin riboside.
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The cross-reactivity of antibodies elicited in rabbits against zeatin riboside, to a wide range of naturally occurring cytokinins, was examined. As well as to zeatin riboside, the antisera cross-reacted to a considerable extent with zeatin, lupinic acid, zeatin-9-glucoside, zeatin riboside
Grain development and endogenous hormones in summer maize (Zea mays L.) submitted to different light conditions.
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Low light is a type of abiotic stress that seriously affects plant growth and production efficiency. We investigated the response mechanisms of summer maize to low light by measuring the changes in endogenous hormones in the grains and during grain filling in summer maize at different light
Exogenously applied spermidine alleviates photosynthetic inhibition under drought stress in maize (Zea mays L.) seedlings associated with changes in endogenous polyamines and phytohormones.
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Drought stress (DS) is a major environmental factor limiting plant growth and crop productivity worldwide. It has been established that exogenous spermidine (Spd) stimulates plant tolerance to DS. The effects of exogenous Spd on plant growth, photosynthetic performance, and chloroplast
Classification and characterization of unknown cytokinins into essential types by in-source collision-induced dissociation electrospray ionization ion trap mass spectrometry.
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BACKGROUND
Mass spectrometry is effective for determination of cytokinins, which are bioactive compounds with an adenine-core structure. However, it is difficult to characterize any cytokinin compound without the relevant standard or known molecular structure information. With a limited number of
Regulators of cell division in plant tissues : XXVIII. Metabolites of zeatin in sweet-corn kernels: Purifications and identifications using high-performance liquid chromatography and chemical-ionization mass spectrometry.
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The cytokinins in certain fractions prepared from extracts of immature sweet-corn (Zea mays L.) kernels using polystyrene ion-exchange resins have been further investigated. Cytokinins active in the radish cotyledon bioassay were purified from these fractions and identified as
O-glucosylation of cis-zeatin in maize. Characterization of genes, enzymes, and endogenous cytokinins.
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trans-Zeatin is a major and ubiquitous cytokinin in higher plants. cis-Zeatin has traditionally been viewed as an adjunct with low activity and rare occurrence. Recent reports of cis-zeatin and its derivatives as the predominant cytokinin components in some plant tissues may call for a different
A highly selective biosensor with nanomolar sensitivity based on cytokinin dehydrogenase.
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We have developed a N6-dimethylallyladenine (cytokinin) dehydrogenase-based microbiosensor for real-time determination of the family of hormones known as cytokinins. Cytokinin dehydrogenase from Zea mays (ZmCKX1) was immobilised concurrently with electrodeposition of a silica gel film on the surface
Specification of cortical parenchyma and stele of maize primary roots by asymmetric levels of auxin, cytokinin, and cytokinin-regulated proteins.
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In transverse orientation, maize (Zea mays) roots are composed of a central stele that is embedded in multiple layers of cortical parenchyma. The stele functions in the transport of water, nutrients, and photosynthates, while the cortical parenchyma fulfills metabolic functions that are not very
Roles of carbohydrate supply and phytohormones in maize kernel abortion.
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Kernels at the ear tip of field grown maize (Zea mays L.) often abort during flowering, resulting in significant yield loss. The objective of this study was to determine if abortion is initiated by an inadequate supply of carbohydrates for growth of ear tip kernels, and/or by a hormonal signal.
Plant Density and Nitrogen Supply Affect the Grain-Filling Parameters of Maize Kernels Located in Different Ear Positions.
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Although yield output of maize (Zea mays L.) has improved markedly over the last century, procedures for improving the grain-filling process remain elusive. Our aim in this study was to relate grain-filling variation in maize (including kernels in apical and middle positions in the ears) to
Disruption of Maize Kernel Growth and Development by Heat Stress (Role of Cytokinin/Abscisic Acid Balance).
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Temperature stress during kernel development affects maize (Zea mays L.) grain growth and yield stability. Maize kernels (hybrid A619 x W64A) were cultured in vitro at 3 d after pollination and either maintained at 25[deg]C or transferred to 35[deg]C for 4 or 8 d, then returned to 25[deg]C until
Hormonal status of maize initial explants and of the embryogenic and non-embryogenic callus cultures derived from them as related to morphogenesis in vitro.
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Endogenous hormone levels (indole-3-acetic acid [IAA], abscisic acid [ABA], gibberellins (1, 3) and (20) [GAs], zeatin/zeatin riboside [Z/ZR] and N(6)[Delta(2)-isopentenyl] adenine/N(6)[Delta(2)-isopentenyl] adenosine; [iP/iPA]) were analysed in immature maize zygotic embryos of two maize (Zea mays
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