[Construction of expression vector driven by tobacco root-specific promoter and transformation into Rhodiola hairy root].

OBJECTIVE

To construct root-specific promoter of plant expression vector and transformate into Rhodiola hairy root.

METHODS

The expression vector pCA-Tob7: UGTR, driven by tobacco root-specific promoter TobRB7, was constructed from pCAM-BIA1301 by substituting the CaMV 35S promoter and GUS gene with TobRB7 and UGTR(a glycosyltransferase gene), respectively. The pCA-Tob7: UGTR vector was introduced into Agrobacterium tumefaciens strain, and the hairy root of Rhodiola were then transformed.

RESULTS

Some Kanamycin resistant plants were positive,which indicated that the expression vector had integrated into Rhodiola hairy root genome.

CONCLUSIONS

Root-specific promoter of plant expression vector is constructed and the hairy root of Rhodiola are transformed for future research.