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Inflammatory Bowel Diseases 2011-Feb

Seroreactivity against glycolytic enzymes in inflammatory bowel disease.

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Krækjan er vistuð á klemmuspjaldið
Nathalie Vermeulen
Severine Vermeire
Ingrid Arijs
Georges Michiels
Vera Ballet
Rita Derua
Etienne Waelkens
Leentje Van Lommel
Frans Schuit
Paul Rutgeerts

Lykilorð

Útdráttur

BACKGROUND

Patients with inflammatory bowel disease (IBD) carry autoantibodies such as perinuclear antineutrophil cytoplasmic antibodies. The aim of the current study was to further characterize the immune reactivity in IBD.

METHODS

We used an immunoproteomic approach with extracts from granulocytes and serum from ulcerative colitis (UC) patients and controls to identify target antigens. By means of Western blot analysis, we screened 60 UC and 60 Crohn's disease (CD) patients, 60 diseased, and 60 healthy controls for the antibodies. We performed gene array experiments on RNA extracted from colonic mucosal biopsies from 42 IBD patients and six controls.

RESULTS

We identified aldolase A, phosphoglycerate mutase, alpha-enolase, triose-phosphate isomerase, and malate dehydrogenase as target antigens in IBD. Seroreactivity to at least one of these five antigens was detected in 53.3% of UC patients, 38.3% of CD patients, and 8.3% of controls. Seroreactivity to at least two antigens was detected in 16.7% of UC patients, 11.7% of CD patients, and none of the controls. Gene array experiments showed a significant upregulation of aldolase A, phosphoglycerate mutase, alpha-enolase, and pyruvate kinase mRNA in biopsies from IBD patients, but not controls. UC and CD patients also showed enhanced expression of hypoxia-inducible factor-1, a transcription factor that induces expression of glycolytic enzymes.

CONCLUSIONS

IBD patients show strong seroreactivity toward enzymes involved in the glycolysis. IBD patients also have increased colonic mRNA expression of glycolytic enzymes, which is triggered by hypoxia through the transcription factor HIF-1.

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