[Speciation analysis of cadmium in cadmium-enriched plants with SE-HPLC combined with ICP-MS and ESI-MS].

Different species of ferns (pyrrosia lingua, Lepisorus thunbergianus, Lycopodium japonicum, Elaphoglossum yoshinagae and Woodwardia japonica) were cultivated using different species of cadmium as cultivation solution. The results showed that there were differences in enrichment amounts of cadmium in different parts of plants (amounts in root > in stem > in leaves). And it was found that different species of cadmium could induce synthesization of plant--chelated peptides (PCs) with different extents. And then PCs was coordinated with cadmium to decrease the toxicity from cadmium. There are some kinds of peptides ligands found in plants, which are mainly PC3, iso-PC3(betaAla) and iso-PC2(betaAla). In the present paper, the distribution of cadmium in different parts of ferns was studied after the ferns were intimidated with different species of cadmium. And the species of PCs-Cd coordination compounds were also investigated to explain the detoxification mechanism aroused by it.