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PLoS ONE 2016

Two Subclasses of Differentially Expressed TPS1 Genes and Biochemically Active TPS1 Proteins May Contribute to Sugar Signalling in Kiwifruit Actinidia chinensis.

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Krækjan er vistuð á klemmuspjaldið
Charlotte Voogd
Lara A Brian
Erika Varkonyi-Gasic

Lykilorð

Útdráttur

Trehalose metabolism and its intermediate trehalose-6-phosphate (T6P) are implicated in sensing and signalling sucrose availability. Four class I TREHALOSE-6-PHOSPHATE SYNTHASE (TPS1) genes were identified in kiwifruit, three of which have both the TPS and trehalose-6-phosphate phosphatase (TPP) domain, while the fourth gene gives rise to a truncated transcript. The transcript with highest sequence homology to Arabidopsis TPS1, designated TPS1.1a was the most highly abundant TPS1 transcript in all examined kiwifruit tissues. An additional exon giving rise to a small N-terminal extension was found for two of the TPS1 transcripts, designated TPS1.2a and TPS1.2b. Homology in sequence and gene structure with TPS1 genes from Solanaceae suggests they belong to a separate, asterid-specific class I TPS subclade. Expression of full-length and potential splice variants of these two kiwifruit TPS1.2 transcripts was sufficient to substitute for the lack of functional TPS1 in the yeast tps1Δ tps2Δ mutant, but only weak complementation was detected in the yeast tps1Δ mutant, and no or very weak complementation was obtained with the TPS1.1a construct. Transgenic Arabidopsis lines expressing kiwifruit TPS1.2 under the control of 35S promoter exhibited growth and morphological defects. We investigated the responses of plants to elevated kiwifruit TPS1 activity at the transcriptional level, using transient expression of TPS1.2a in Nicotiana benthamiana leaves, followed by RNA-seq. Differentially expressed genes were identified as candidates for future functional analyses.

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