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agar/brjóstakrabbamein
Krækjan er vistuð á klemmuspjaldið
Bls 1 frá 713 niðurstöður
Selection of brain metastasis-initiating breast cancer cells determined by growth on hard agar.
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An approach that facilitates rapid isolation and characterization of tumor cells with enhanced metastatic potential is highly desirable. Here, we demonstrate that plating GI-101A human breast cancer cells on hard (0.9%) agar selects for the subpopulation of metastasis-initiating cells. The
Differential effects of estrogen and antiestrogen on in vitro clonogenic growth of human breast cancers in soft agar.
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Of 534 human primary breast cancers provided for clonogenic assay in vitro, 276 (51.7%) developed distinctive colony formation by the soft-agar method. Estrogen receptors (ERs) were assayed by dextran-coated charcoal methods. A total of 65 (23.7%) of 274 breast cancers responded to added 10 nM 17
Colonies formed in agar from human breast cancer and their identification as T-lymphocytes.
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Single cell suspensions prepared from human breast cancer specimens by collagenase digestion were cultured in soft agar with phytohemagglutinin-stimulated human lymphocyte-conditioned medium (PHA-LCM). In 6 of 10 different tumors, PHA-LCM-dependent clonal growth was develop. After 12-14 days of
Promotion by prolactin of the growth of human breast neoplasms cultured in vitro in the soft agar clonogenic assay.
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The role of prolactin (PRL) in supporting the growth of human breast cancer is still unclear. The ability to grow primary breast cancer specimens in the soft agar clonogenic assay in the absence of serum gave us the opportunity to evaluate the growth-promoting effect of PRL and to compare it to that
Endocrine therapy testing of human breast cancers in the soft agar clonogenic assay.
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The human tumor soft agar cloning assay has been used to assess the biological effects of cytotoxic drugs and other agents on human cancers. In this study we have examined the effects of two hormonal agents, tamoxifen (Tam) and medroxyprogesterone acetate (MPA), on colony growth of the MCF-7 human
Growth factor involvement in the multihormonal regulation of MCF-7 breast cancer cell growth in soft agar.
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The hormone dependency of the MCF-7 breast cancer cell line, while extensively tested in liquid culture, has not been previously evaluated under conditions of anchorage-independent growth in serum-free media. Using the soft agar clonogenic assay, we demonstrate that physiologically relevant
Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells.
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Given the inherent difficulties in investigating the mechanisms of tumor progression in vivo, cell-based assays such as the soft agar colony formation assay (hereafter called soft agar assay), which measures the ability of cells to proliferate in semi-solid matrices, remain a hallmark of cancer
Quantitation of estrogen receptors in human breast cancer by agar gel electrophoresis.
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Samples from human primary and metastatic mammary carcinomas were examined for the presence of specific estrogen-binding proteins. Cytosols were incubated with [3H]estradiol-17beta with and without an excess of unlabelled estradiol or estrogen antagonist. After incubation, the mixtures were treated
Soft agar clonogenic assay in human breast cancer.
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Relationship between histology, cloning efficiencies and estrogen receptors was studied in the group of breast cancer patient. Mechanical disaggregation gave poor cellular yields since only in 66% cells were ready for the bioassay. Comparison between clonogenic assay, histology and estrogen status
Direct cloning of human breast cancer in soft agar culture.
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A human tumor cloning system has been utilized to grow human breast carcinoma. A total of 225 specimens have been placed in culture. One hundred thirty-two were from primary chest cancer specimens and 93 were from metastatic lesions. Of these, 71% of the primary breast carcinomas and 75% of
Identification of mesothelial cell clusters in agar cultures of effusions from patients with breast cancer.
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Adhesion to type V collagen and cloning efficiency in agar of 8701-BC breast cancer cells.
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WISP3 and RhoC guanosine triphosphatase cooperate in the development of inflammatory breast cancer.
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BACKGROUND
Inflammatory breast cancer (IBC) is the most lethal form of locally advanced breast cancer. We found concordant and consistent alterations of two genes in 90% of IBC tumors when compared to stage matched, non-IBC tumors: overexpression of RhoC GTPase and loss of WISP3. Further work
Metastasis tumor-associated protein 2 enhances metastatic behavior and is associated with poor outcomes in estrogen receptor-negative breast cancer.
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Metastasis remains a major clinical problem in breast cancer. One family of genes previously linked with metastasis is the metastasis tumor-associated (MTA) family, with members MTA1 enhancing and MTA3 inhibiting cancer metastasis. We have previously found that MTA2 enhances anchorage-independent
Tumor suppressive function of Matrin 3 in the basal-like breast cancer
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Background: Basal-like breast cancer (BLBC) or triple-negative breast cancer (TNBC) is an aggressive and highly metastatic subtype of human breast cancer. The present study aimed to elucidate the potential tumor-suppressive function of
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