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Concanavalin A in vivo: induction of hemorrhagic skin lesions (Arthus-like reactions) in mice.

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Concanavalin A injected into the skin of mice induced the formation of hemorrhagic Arthus-like lesions. No lesions resulted if concanavalin A was adsorbed with insoluble (a2)- macroglobulin or if mice were first treated with nitrogen mustard. The ability of concanavalin A, phytohemagglutinin, or

Lymphocyte concanavalin A capping in hereditary cerebral haemorrhage with amyloidosis--Dutch type.

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Lymphocyte capping with concanavalin A was studied in 11 patients with hereditary cerebral haemorrhage with amyloidosis (Dutch type) and 10 controls. No difference in capping was found between patients and controls. Abnormal lymphocyte concanavalin A capping has been reported in patients with the

Transgenic prolactin-/- mice: effect of trauma-hemorrhage on splenocyte functions.

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Prolactin (PRL) is involved in the regulation of immune functions under normal and pathological conditions. Trauma-hemorrhage (T-H) produces profound immunosuppression in male mice but not in proestrus female mice. Administration of PRL in males after T-H, however, restores immune functions. In this

Cell-mediated immune response and IL-2 production in white-tailed deer experimentally infected with hemorrhagic disease viruses.

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Hemorrhagic disease, caused by various serotypes of two closely related orbivirus serogroups, the epizootic hemorrhagic disease viruses (EHDV) and the bluetongue viruses (BTV), is a major cause of morbidity and mortality in white-tailed deer (WTD) in the United States. Despite the importance of

The potential role of T-cells and their interaction with antigen-presenting cells in mediating immunosuppression following trauma-hemorrhage.

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OBJECTIVE Trauma-hemorrhage results in depressed immune responses of antigen-presenting cells (APCs) and T-cells. Recent studies suggest a key role of depressed T-cell derived interferon (IFN)-g in this complex immune cell interaction. The aim of this study was to elucidate further the underlying

Precipitating antibodies to non-treated dengue type 2 viral antigens in sera of Thai hemorrhagic fever patients by crossed immunoelectrophoresis.

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Crossed immunoelectrophoresis of dengue type 2 virus revealed at least two precipitating antigens which shared some antigenic determinants. Glycoprotein components of both antigens were detected by binding to concanavalin A. Sera from dengue hemorrhagic fever patients showed precipitating antibodies

In vitro replication of epizootic hemorrhagic disease and bluetongue viruses in white-tailed deer peripheral blood mononuclear cells and virus-cell association during in vivo infections.

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In vitro and in vivo infections were conducted to determine if the epizootic hemorrhagic disease (EHD) and bluetongue (BT) viruses would replicate in peripheral blood mononuclear (PBM) cells of white-tailed deer (Odocoileus virginianus). All of the North American EHD and BT viruses (EHD virus

[Immunological investigation of late cerebral vasospasm in subarachnoid hemorrhage. (Part 1)].

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Although the occurrence of so-called late cerebral vasospasm after subarachnoid hemorrhage (SAH) due to ruptured cerebral aneurysm is well-known, its etiology still remains obscure. This time, the authors investigated the etiology by immunological research. Following results were obtained, Out of 13

Impaired in vitro proliferative response of suppressor lymphocytes in patients with subarachnoid haemorrhage from ruptured intracranial aneurysm.

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Proliferative response to mitogens concanavalin A, phytohemagglutinin and pokeweed mitogen, and other chosen indicators of the activity of the immune system were assayed in peripheral blood mononuclear cells isolated from blood of patients with subarachnoid haemorrhage from ruptured aneurysm.

Intraocular hemorrhage causes retinal vascular dysfunction via plasma kallikrein.

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OBJECTIVE Retinal hemorrhages occur in a variety of sight-threatening conditions including ocular trauma, high altitude retinopathy, and chronic diseases such as diabetic and hypertensive retinopathies. The goal of this study is to investigate the effects of blood in the vitreous on retinal vascular

Suppression of activation and costimulatory signaling in splenic CD4+ T cells after trauma-hemorrhage reduces T-cell function: a mechanism of post-traumatic immune suppression.

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Reduced immune function is frequently a consequence of serious injury such as trauma-hemorrhage (T-H). Injury may lead to reduced T-cell activation, resulting in decreased engagement of costimulatory molecules after antigen recognition and in subsequent immunological compromise and anergy. We

In vitro depression of the mitogenic response of lymphocytes from turkeys infected with hemorrhagic enteritis virus.

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A depression in the mitogenic response of lymphocytes was demonstrated in turkeys inoculated with hemorrhagic enteritis virus. Blood samples were collected (in heparin) once a week, beginning 1 week after the turkeys were inoculated. The whole blood assay was used to study lymphoblastogenesis.

Concanavalin A and polyvinyl butyral use as a potential dengue electrochemical biosensor.

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Immobilization of concanavalin A on gold electrode by means of gold nanoparticles and polyvinyl butyral was carried out and investigated by cyclic voltammetry and electrochemical impedance spectroscopy. The system was tested with sera from patients infected by dengue fever (DF) and dengue

Purification of the glycoprotein G from viral haemorrhagic septicaemia virus, a fish rhabdovirus, by lectin affinity chromatography.

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A new method for the isolation of glycoprotein G from viral haemorrhagic septicaemia virus (VHSV), a fish rhabdovirus, was developed by using affinity chromatography with immobilized Concanavalin A (ConA). The glycoprotein G was isolated from detergent solubilized concentrated virions and from

A reevaluation of the concanavalin A induced suppressor cell assay in man.

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Several methods are used to evaluate the in vitro suppressor function of human peripheral blood lymphocytes (PBL). Recently however, the validity of these assays has been questioned, because possible artefacts may lead to misinterpretation of the results. A reevaluation of these assays has been

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