silica/arabidopsis

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Highly efficient analysis of underivatized carbohydrates using monolithic-silica-based capillary hydrophilic interaction (HILIC) HPLC.

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A polyacrylamide (PAAm)-modified monolithic silica capillary column of increased phase ratio, 200T-PAAm, for hydrophilic interaction liquid chromatography (HILIC) was prepared. The column showed high separation efficiency, with a theoretical plate height H = 7-20 microm at a linear velocity, u = 1-7

Monolithic silica-based capillary reversed-phase liquid chromatography/electrospray mass spectrometry for plant metabolomics.

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Application of C18 monolithic silica capillary columns in HPLC coupled to ion trap mass spectrometry detection was studied for probing the metabolome of the model plant Arabidopsis thaliana. It could be shown that the use of a long capillary column is an easy and effective approach to reduce

Protocol: high throughput silica-based purification of RNA from Arabidopsis seedlings in a 96-well format.

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The increasing popularity of systems-based approaches to plant research has resulted in a demand for high throughput (HTP) methods to be developed. RNA extraction from multiple samples in an experiment is a significant bottleneck in performing systems-level genomic studies. Therefore we have

Silica nanoparticle phytotoxicity to Arabidopsis thaliana.

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The phytotoxicity of silica nanoparticles (SiNPs) was evaluated as a function of particle size (14, 50, and 200 nm), concentration (250 and 1000 mg L(-1)), and surface composition toward Arabidopsis thaliana plants grown hydroponically for 3 and 6 weeks. Reduced development and chlorosis were

A simple plant gene delivery system using mesoporous silica nanoparticles as carriers.

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A facile DNA delivery method would greatly facilitate studies of plant functional genomics. However, plant cell walls limit the utilization of nanoparticles on plant research. Here, we employed functionalized mesoporous silica nanoparticles (MSNs) to develop a MSN-mediated plant transient gene

Callose-associated silica deposition in Arabidopsis.

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The mechanism of biological silicification in plants remains to be elucidated. There are strong arguments supporting a role for the plant extracellular matrix and the β-1-3-glucan, callose, has been identified as a possible template for silica deposition in the common horsetail, Equisetum arvense.

Delivery of Abscisic Acid to Plants Using Glutathione Responsive Mesoporous Silica Nanoparticles.

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An intracellular glutathione (GSH) responsive phytochemical delivery system based on thiol gated mesoporous silica nanoparticles (MSNs) was developed and tested on the model plant Arabidopsis thaliana. In the present study, monodispersed MSNs with particle diameters of ~20 nm and pore sizes of ~2.87

Exocyst Subunit EXO70H4 Has a Specific Role in Callose Synthase Secretion and Silica Accumulation.

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Biogenesis of the plant secondary cell wall involves many important aspects, such as phenolic compound deposition and often silica encrustation. Previously, we demonstrated the importance of the exocyst subunit EXO70H4 for biogenesis of the trichome secondary cell wall, namely for deposition of the

Is callose required for silicification in plants?

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The cell wall polymer callose catalyses the formation of silica in vitro and is heavily implicated in biological silicification in Equisetum (horsetail) and Arabidopsis (thale cress) in vivo Callose, a β-1,3-glucan, is an ideal partner for silicification, because its amorphous structure and

Stabilization of RNA through absorption by functionalized mesoporous silicate nanospheres.

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The potential for encapsulating RNA within tunable, semi-permeable structures for storage and transportation purposes offers an interesting approach to the reduction of stringent storage requirements that often hamper the field application of genetic analysis methods. In this study, we assessed the

Ligand migration in nonsymbiotic hemoglobin AHb1 from Arabidopsis thaliana.

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AHb1 is a hexacoordinated type 1 nonsymbiotic hemoglobin recently discovered in Arabidopsis thaliana. To gain insight into the ligand migration inside the protein, we studied the CO rebinding kinetics of AHb1 encapsulated in silica gels, in the presence of glycerol. The CO rebinding kinetics after

Calcium phosphate in plant trichomes: the overlooked biomineral.

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UNASSIGNED Calcium phosphate was unknown as a plant biomineral until recently reported in Neotropical Loasaceae. Here, we demonstrate its widespread occurrence in the trichomes of several plant families, including Brassicaceae. Calcium phosphate is the primary biomineral in, e.g., the bones and

Chemistry of carotenoid neutral radicals.

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Proton loss from the carotenoid radical cations (Car(+)) to form neutral radicals (#Car) was investigated by numerous electrochemical, EPR, ENDOR and DFT studies described herein. The radical cation and neutral radicals were formed in solution electrochemically and stabilized on solid silica-alumina

Inhibition of reproductive growth of Arabidopsis in airtight vessels.

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Airtight vessels have various advantages for space experiments. However, Arabidopsis thaliana plants scarcely produced seeds when grown in such vessels. The mechanism by which reproductive growth is inhibited in airtight vessels was studied. The length of the flower stalk was shorter when the plants

Different roles of protein dynamics and ligand migration in non-symbiotic hemoglobins AHb1 and AHb2 from Arabidopsis thaliana.

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The ligand rebinding kinetics after photolysis of the CO complexes of Arabidopsis thaliana hemoglobins AHb1 and AHb2 in solution show very different amplitudes in the geminate phase, reflecting different migration pathways of the photodissociated ligand in the system of internal cavities accessible

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