water/phosphatase

Problems caused by cyanobacteria are common around the world and also in raw water sources of drinking water treatment plants. Strains belonging to genera Microcystis, Anabaena and Planktothrix produce potent hepatotoxins, the microcystins. Laboratory and pilot scale studies have shown that

Freeze-drying of enzymes in case of water-binding and non-water-binding substrates.

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Enzymes typically have a critical instability, which dominates both formulation and process development. In this paper, the ability to preserve the enzyme activity during freeze-drying was investigated for both water-binding and non-water-binding substrates. For this purpose, acid phosphatase was

Role of metal ions in Escherichia coli alkaline phosphatase. A study of the metal-water interaction by nuclear relaxation rate measurements on water protons.

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The binding of metal to alkaline phosphatase from Escherichia coli and the binding of water and orthophosphate to the Me-2+-enzyme binary complex have been examined by water proton relaxation rate (PRR) measurements. Titration of the three paramagnetic metals, Mn2+, Cu2+, and Co2+, into apoalkaline

Utilizing ultrafiltration to remove alkaline phosphatase from clinical analyzer water.

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Alkaline phosphatase (ALP) conjugated to antibodies is often used in enzyme immunoassays (EIAs). These assays are notably sensitive to experimental conditions. A possible source of interference is bacterial ALP, which is released when bacterial contamination occurs in clinical analyzers. Preliminary

Hydrolysis of phosphohistidine in water and in prostatic acid phosphatase.

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Calculation of the free energy profile for hydrolysis of phosphohistidine using ONIOM methodology indicates a much tighter transition state in the enzyme active site compared to that in explicit water and elucidates the role of active site residues in catalysis.

Experimental rat model for acute tubular injury induced by high water hardness and high water fluoride: efficacy of primary preventive intervention by distilled water administration.

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High water hardness associated with high water fluoride and the geographical distribution of Chronic Kidney Disease of unknown etiology (CKDu) in Sri Lanka are well correlated. We undertook this study to observe the effects of high water hardness with high fluoride on kidney and liver

Alkaline phosphatase activities of anAnabaena sp. from deep-water rice.

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Anabaena sp. grew with mono- and di-ester phosphate compounds as sources of phosphate, indicating the presence of phosphomonoesterase (PMEase) and phosphodiesterase (PDEase) activities. Cell-bound PMEase and PDEase activities were detected during growth in 0.5 and 10 mg PO4l(-1) only when the

[Effect of sulfur water baths on phosphatases in ankylosing spondylitis].

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Nutrient regeneration mediated by extracellular enzymes in water column and interstitial water through a microcosm experiment.

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In coastal lakes the role of microorganisms in driving nutrients regeneration at different water depths and in sediments is not yet fully understood. The dynamics of microbial (algal and bacterial) abundance and bacterial activities involved in organic matter transformation were measured, together

Phosphate (oxygen)-water exchange reaction catalyzed by human prostatic acid phosphatase.

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Conclusive evidence is presented that an acid phosphatase catalyzes phosphate (oxygen)-water exchange. Studies conducted with human prostatic acid phosphatase by two independent methods have established that, despite earlier reports to the contrary, the enzyme catalyzes an exchange reaction between

Alkaline phosphatase activity in the phosphorus-limited southern Chinese coastal waters.

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Three fractions of alkaline phosphatase activity (APA), including phytoplankton APA (phyto-APA), bacterial APA (bact-APA), and free-APA, were examined in the sea surface microlayer (SML) and the subsurface water (SSW) from Daya Bay, Guishan Island, and Guanghai Bay of southern China. Relationships

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