Selective photodynamic effects on breast cancer cells provided by P123 Pluronic®-based nanoparticles modulating hypericin delivery.
Parole chiave
Astratto
BACKGROUND
Breast cancer is the most relevant kind of cancer and the second cause of cancer- related deaths among women in general. Currently, there is no effective treatment for breast cancer although advances in its initial diagnosis and treatment available. Therefore, the value of novel anti-tumor therapeutic modalities remains an immediate unmet need in clinical practice. Following our previous work regarding to the properties of the Pluronics with different photosensitizer (PS) for photodynamic therapy (PDT), in this study we aimed to evaluate the efficacy of supersaturated hypericin (HYP) encapsulated on Pluronic® P123 (HYP/P123) against breast cancer cells (MCF-7) and non-tumorigenic breast cells (MCF-10A).
METHODS
Cell internalization e subcellular distribution of HYP/P123 was confirmed by fluorescence microscopy. The phototoxicity and citototoxicity of HYP/P123 was assessed by trypan blue exclusion assay in the presence and absence of light. Long-term cytotoxicity was performed by clonogenic assay. Cell migration was determined by the wound-healing assay. Apoptosis and necrosis assays were performed by annexin V-FITC/propidium Iodide (PI) by fluorescence microscopy.
RESULTS
Our results showed that HYP/P123 micelles had high stability and high rates of binding to cells, which resulted in their selective internalization in MCF-7, indicating their potential to permeate the membrane of these cells. Moreover, HYP/P123 micelles accumulated in mitochondria and endoplasmic reticulum organelles, resulting in photodynamic cell death by necrosis. Additionally, HYP/P123 micelles show effective and selective time- and dose dependent phototoxic effects on MCF-7 cells but little damage to MCF-10A cells. HYP/P123 micelles inhibited the generation of cellular colonies, indicating a possible capability to prevent the recurrence of breast cancer. We also showed that HYP/P123 micelles inhibit the migration of tumor cells, possibly by decreasing their ability to form metastases CONCLUSION: Taken together, the results presented here indicate a potentially useful role of HYP/P123 micelles as a platform for HYP delivery to more specifically and effectively treat human breast cancers through photodynamic therapy, suggesting they are worthy of in vivo preclinical evaluations.
