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actinomycin d/solanum tuberosum
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Pagina 1 a partire dal 22 risultati
Potato spindle tuber viroid. XIII. Inhibition of replication by actinomycin D.
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Effect of actinomycin D on potato virus X multiplication in Datura stramonium leaves.
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The regulation of synthesis and storage of chymotrypsin inhibitor I in leaves of potato and tomato plants.
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The synthesis and accumulation of chymotrypsin inhibitor I in tomato leaflets is induced by detachment, or by destruction of petiole phloem by steam when followed by incubation of the leaflets in light. The induction process with detached tomato leaflets is similar to that found with detached potato
Induction of Rishitin and Lubimin Synthesis in Potato Tuber Slices by Non-Specific Elicitors - Role of Gene Derepression.
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The stress metabolites rishitin and lubimin accumulate at relatively low concentrations (5-20 ppm) in potato tuber slices subjected to various cell-disruptive treatments including heavy metal salts, sulfhydryl reagents, metabolic inhibitors, detergents, ultraviolet light and lysosomal enzymes.
Ribosomal RNA synthesis in newly sliced discs of potato tuber.
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A burst of ribosomal RNA synthesis is induced in potato tissue by slicing, and continues at a decreasing rate for about 12 hours. Ribosomal RNA synthesis in potato discs is sensitive to puromycin, in contrast to non-ribosomal RNA synthesis. Thus, the influence of puromycin on total RNA synthesis is
Studies on the induction of cinnamic acid 4-hydroxylase in potato tuber.
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The change in activity of cinnamic acid 4-hydroxylase (CA4H) in potato parenchyma tissue exposed to various conditions has been examined. Maximum induction of CA4H activity was obtained at 18 hr of incubation. Though CA4H induction can occur in dark, over 100% increase in enzyme activity was
Synthesis of (+) and (-) RNA molecules of potato spindle tuber viroid (PSTV) in isolated nuclei and its impairment by transcription inhibitors.
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Transcription studies with highly purified potato cell nuclei in combination with a 'transcription-hybridization analysis' unequivocally demonstrate that the nucleus is the subcellular site where the entire process of PSTV replication takes place. Inhibition experiments with actinomycin D and
[Carrier-mediated uptake of L-leucine at the points of growth separated from potato tubers].
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The uptake of 14C-L-leucine by the points of growth separated from potato tubers was studied. The pH optimum of the process was about 7.0. The leucine transport over a wide concentration range was biphasic. For descriptive convenience the isotherm was divided into two segments, both of which obeyed
Fat metabolism in higher plants. XXXIV. Development of fatty acid synthetase as a function of protein synthesis in aging potato tuber slices.
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Experiments with inhibitors of protein synthesis (actinomycin D, puromycin, actidione) showed that the increase and the change in fatty acid synthetase activity, observed during the aging of potato disks, were accompanied by and related to a temporary rise in the rate of protein and RNA synthesis.
Wound-Induced RNase Activity in Sweet Potato : EVIDENCE FOR REGULATION AT TRANSCRIPTION.
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Upon wounding of sweet potato (Ipomea batatas, Lam. var. Puerto Rico) RNase activity increases rapidly following a 4-hour lag, peaks in 24 hours, and then declines. Cycloheximide inhibits induction indicating that increased activity is probably due to de novo synthesis. The half-time (t(0.5)) for
Increase in ribonuclease activity following mechanical damage to leaf and tuber tissues of Solanum tuberosum L.
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Major increases occurred in the capacity of damaged potato leaf and tuber tissues to hydrolyse ribonucleic acid whilst relatively minor increases were found in the activity of acid phosphomonoesterase and acid phosphodiesterase. Partial purification of homogenates by gel filtration on Sephadex G-100
Wound-induced phenylalanine ammonia-lyase in potato tuber tissue. Development of enzyme activity and effects of antibiotics.
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Phenylalanine ammonia-lyase [EC 4.3.1.5.] activity increased rapidly after a 3-hr lag period in potato tuber (Solanum tuberosum L. cv. May Queen) disks incubated in a suitable medium in the dark at 25 degrees. The activity reached a maxinum after incubation for about 40 hr. The effects of
Changes in potato tuber invertase and its endogenous inhibitor after slicing, including a study of assay methods.
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The increase in the invertase activity of extracts from freshly cut potato (Solanum tuberosum L.) by "foaming," caused by selective denaturation of an endogenous invertase inhibitor, did not occur in extracts made from thin disks 2 days after slicing. Rather, foaming such extracts decreased
Increased Chorismate Mutase Levels as a Response to Wounding in Solanum tuberosum L. Tubers.
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Discs excised from Solanum tuberosum L. cv White Rose tubers demonstrated a 4.5-fold increase in chorismate mutase activity 48 hours after excision. Incubation in the presence of cycloheximide (25 micromolar) or actinomycin D (100 micromolar) completely inhibited the wound response suggesting de
Biochemistry of Suberization: omega-Hydroxyacid Oxidation in Enzyme Preparations from Suberizing Potato Tuber Disks.
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A cell-free extract obtained from suberizing potato (Solanum tuberosum L.) tuber disks catalyzed the conversion of 16-hydroxy[G-(3)H]hexadecanoic acid to the corresponding dicarboxylic acid with NADP or NAD as the cofactor, with a slight preference for the former. This omega-hydroxyacid
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