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12 risultati
Lichen distribution and bioindicator tobacco plants give discordant response: a case study from Italy.
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Epiphytic lichen biodiversity (LB) distribution was evaluated in a 1000 km2 area in Tuscany (Central Italy). In the same area, a survey was performed to monitor tropospheric ozone (O3) phytotoxic effects using tobacco plants (Nicotiana tabacum) cv. Bel-W3 (O3 supersensitive) and cv. Bel-B (O3
Incorporation of cytokinin N-benzyladenine into tobacco callus transfer ribonucleic Acid and ribosomal ribonucleic Acid preparations.
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The incorporation of the cytokinin N(6)-benzyladenine into tobacco (Nicotiana tabacum) callus tRNA and rRNA preparations isolated from tissue grown on medium containing either N(6)-benzyladenine-8-(14)C or N(6)-benzyladenine-8-(14)C: benzene-(3)H(G) has been examined. N(6)-benzyladenine was
2'-epi-orobanchol and solanacol, two unique strigolactones, germination stimulants for root parasitic weeds, produced by tobacco.
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Germination stimulants for root holoparasitic weeds broomrapes ( Orobanche and Phelipanche spp.) produced by tobacco ( Nicotiana tabacum L.) were purified and characterized. The root exudates of tobacco contained at least five different stimulants, and LC-MS/MS analyses revealed that four of them
[Transgenic plant regeneration of tobacco (Nicotiana tabacum) haboring mammalian cyp2e1 gene].
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CYP2E1 enzyme encoded by cyp2e1 gene plays an important role in metabolism of heterogeneous organics in mammalian liver cells. The transgenic plant with cyp2e1 can metabolize various low molecular weight organic pollutants. However, it is unclear the mechanism of expression control of cyp2e1 in
Untargeted Metabolomics of Nicotiana tabacum Grown in United States and India Characterizes the Association of Plant Metabolomes With Natural Climate and Geography.
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Climate change and geography affect all the living organisms. To date, the effects of climate and geographical factors on plant metabolome largely remain open for worldwide and local investigations. In this study, we designed field experiments with tobacco (Nicotiana tabacum) in India
Characterization of nigericin-stimulated ATPase from sealed microsomal vesicles of tobacco callus.
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To understand the function and membrane origin of ionophore-stimulated ATPases, the activity of nigericin-stimulated ATPase was characterized from a low-density microsomal fraction containing sealed vesicles of autonomous tobacco (Nicotiana tabacum Linnaeous cv. Wisconsin no. 38) callus. The
An NADPH-Oxidase/Polyamine Oxidase Feedback Loop Controls Oxidative Burst Under Salinity.
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The apoplastic polyamine oxidase (PAO) catalyzes the oxidation of the higher polyamines spermidine and spermine, contributing to hydrogen peroxide (H2O2) accumulation. However, it is yet unclear whether apoplastic PAO is part of a network that coordinates the accumulation of reactive oxygen species
Use of a new tetrazolium-based assay to study the production of superoxide radicals by tobacco cell cultures challenged with avirulent zoospores of phytophthora parasitica var nicotianae
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The relationship between the production of reactive oxygen species and the hypersensitive response (HR) of tobacco (Nicotiana tabacum L. ) toward an incompatible race of the Oomycete Phytophthora parasitica var nicotianae has been investigated. A new assay for superoxide radical (O2-) production
Two Novel Motifs of Watermelon Silver Mottle Virus NSs Protein Are Responsible for RNA Silencing Suppression and Pathogenicity.
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The NSs protein of Watermelon silver mottle virus (WSMoV) is the RNA silencing suppressor and pathogenicity determinant. In this study, serial deletion and point-mutation mutagenesis of conserved regions (CR) of NSs protein were performed, and the silencing suppression function was analyzed through
Degradation of low molecular weight volatile organic compounds by plants genetically modified with mammalian cytochrome P450 2E1.
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Cytochrome P450 2E1 (CYP2E1) is a key enzyme in the mammalian metabolism of several low molecular weight volatile organic compounds (VOCs), such as trichloroethylene (TCE), vinyl chloride (VC), carbon tetrachloride (CT), benzene, chloroform, and bromodichloromethane (BDCM), which are all common
Specific plant DNA adducts as molecular biomarkers of genotoxic atmospheric environments.
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The general purpose of this study was to determine whether the formation of DNA addition products ('adducts') in plants could be a valuable biomarker of genotoxic air pollution. Plants from several species were exposed to ambient atmosphere at urban and suburban sites representative of different
Identification of Benzyloxy Carbonimidoyl Dicyanide Derivatives as Novel Type III Secretion System Inhibitors via High-Throughput Screening.
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The type III secretion system (T3SS) in many Gram-negative bacterial pathogens is regarded as the most critical virulence determinant and an attractive target for novel anti-virulence drugs. In this study, we constructed a T3SS secretion reporter containing the β-lactamase gene fused with a
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