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12 risultati
Improved detection of oligoclonal IgG in cerebrospinal fluid by isoelectric focusing in agarose, double-antibody peroxidase labeling, and avidin-biotin amplification.
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To demonstrate oligoclonal IgG bands (I) in unconcentrated cerebrospinal fluid, we used isoelectric focusing in agarose followed by protein transfer to cellulose nitrate membrane, double-antibody peroxidase labeling, and avidin-biotin amplification. I can be reliably seen after isoelectric focusing
Biotin and Acetazolamide for Treatment of an Unusual Child With Autism Plus Lack of Nail and Hair Growth.
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BACKGROUND
Patients with autism spectrum disorder and developmental delay or encephalopathy rarely demonstrate no or negligible hair and nail growth, suggesting a biotin-responsive clinical disorder.
METHODS
A ten-year-old girl presented with features of autism spectrum disorder, isolated headaches,
Biotin-responsive depression during hyperalimentation.
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A patient with multiple enteric fistulae, after months of parenteral hyperalimentation, developed, severe depression accompanied by delirium, dermatitis, pallor, paresthesia, nausea, vomiting, anorexia, and headaches. His symptoms improved after treatment with parenteral biotin. Biotin-deficiency
Vascular head pain selectively activates ventrolateral periaqueductal gray in the cat.
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Electrical stimulation of the superior sagittal sinus is an experimental model of migraine which activates neurons within the upper cervical spinal cord. The ventrolateral periaqueductal gray has been proposed as an integrative centre for the autonomic and behavioural responses to deep pain and also
Simultaneous detection of herpes simplex virus types 1 and 2 by real-time PCR and Pyrosequencing.
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BACKGROUND
Up to 80% of the US adult population has been exposed to herpes simplex virus (HSV) type 1, primarily during childhood. Also, approximately 20% of the US population has contracted genital herpes from HSV-2 infections. Clinical symptoms can present as fever, headache, malaise, myalgia, and
Hypeprolactinemia: still an insidious diagnosis
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Hyperprolactinemia can have different causes: physiological, pharmacological, and pathological. When investigating the etiology of hyperprolactinemia, clinicians need to be aware of several conditions leading to misdiagnosis. The most popular pitfalls are: acute physical and psychological stress,
The induction and characterization of monoclonal antibodies specific to GP of Ebola virus.
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The Ebola virus is highly infectious and characterized of hemorrhagic fever, headache, etc. with high mortality rate. Currently there are neither therapeutic drugs or vaccines against Ebola virus nor fast diagnositic method for the detection of Ebola virus infection. This study reported the
A Rapid Quantitative Chemiluminescence Immunoassay for Vitamin B12 in Human Serum.
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Rosette-forming glioneuronal tumor: pathology case report.
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OBJECTIVE
Rosette-forming glioneuronal tumor is a newly described mixed glial and neuronal tumor. We describe two cases and review the literature to better characterize this entity.
METHODS
Patients were surgically treated, and tumors were diagnosed by light microscopy and immunohistochemistry using
Agarose isoelectric focusing, antiserum immunofixation and silver staining for detection of oligoclonal bands in unconcentrated cerebrospinal fluid.
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Unconcentrated cerebrospinal fluid (CSF) and corresponding serum from 30 patients with multiple sclerosis (MS), 30 with other neurological disease and 30 controls suffering from tension headache or psychoneurosis, were examined for oligoclonal IgG bands by initial separation employing agarose
Reduction of TRPV1 expression in the trigeminal system by botulinum neurotoxin type-A.
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Botulinum neurotoxin type-A (BoNT-A) is clinically used for patients with pain disorders and dystonia. The precise mechanism whereby BoNT-A controls pain remains elusive. Here, we studied how BoNT-A affects the expression of the transient receptor potential vanilloid subfamily member 1 (TRPV1), a
Vitamin neurotoxicity.
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Vitamins contain reactive functional groups necessary to their established roles as coenzymes and reducing agents. Their reactive potential may produce injury if vitamin concentration, distribution, or metabolism is altered. However, identification of vitamin toxicity has been difficult. The only
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