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digoxigenin/arabidopsis thaliana
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5 risultati
Optimization of a digoxigenin-based immunoassay system for gene detection in Arabidopsis thaliana.
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Digoxigenin is derived from a plant steroid hormone digoxin found in the plants Digitalis sp. Digoxigenin has been used successfully in labeling nucleic acids. In this experiment we optimized minimum probe requirement for a nonradioactive digoxigenin-based gene detection system in the model plant
Feasible and reliable quantification of mRNA in Arabidopsis thaliana using optical thin-film biosensor chips.
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mRNA quantification is very important in molecular biological researches. Traditional spectrophotometric method cannot distinguish DNA, rRNA and tRNA species from mRNA. Northern blot can be used for mRNA quantification but is known to be time consuming. To rapidly detect mRNA levels, we developed an
Nonradioactive labeling of large DNA fragments for genome walking, RFLP and northern blot analysis.
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In this report, we present a simple nonradioactive labeling procedure for DNA fragments of high specific labeling density that can be used for a variety of applications. The protocol is based on the universal mono-functional platinum reagent for chemical digoxigenin (DIG) labeling of nucleic acids.
Non-radioactive TRF assay modifications to improve telomeric DNA detection efficiency in plants.
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The length of telomeric DNA is often considered a cellular biomarker of aging and general health status. Several telomere length measuring assays have been developed, of which the most common is the Telomere Restriction Fragment (TRF) analysis, which typically involves the use of radioactively
Identification of programmed cell death related genes in bamboo.
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The event of bamboo flowering and subsequent death of bamboo cells, a rare phenomenon is an interesting model to study gene expression/function in the context of the programmed cell death (PCD) in plant. To identify genes involved in autolytic cell death in bamboo (Bambusa arundinacea/Bambusa bambos
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