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hepatitis/solanum tuberosum
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Pagina 1 a partire dal 52 risultati
Polypeptides of hepatitis B surface antigen produced in transgenic potato.
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Chimeric genes containing the genomic fragments of hepatitis B virus (HBV) coding for middle (M) and major/small (S) surface proteins were constructed and expressed in transgenic potato plants under the control of the cauliflower mosaic virus 35S promoter. Enzyme-linked immunosorbent assay (ELISA)
Antibody responses in mice stimulated by various doses of the potato-derived major surface antigen of hepatitis B virus.
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The ability of potato-derived major surface antigen of hepatitis B virus (P-HBsAg) to elicit antibody responses to different dosages of P-HBsAg ranging from 0.02 to 30 μg administered orally in mice was examined. All immunized groups produced specific serum IgG and fecal IgA antibodies against
Expression and characterization of hepatitis B surface antigen in transgenic potato plants.
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Transgenic potato plants expressing the gene of hepatitis B surface antigen (HBsAg) under the control of the double promoter of 35S RNA of cauliflower mosaic virus (CaMV 35SS) and the promoter of patatin gene of potato tubers have been obtained. Biochemical analysis of the plants was performed. The
Process options in hepatitis B surface antigen extraction from transgenic potato.
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The process conditions for recombinant hepatitis B surface antigen (HBsAg) extraction from transgenic potato were examined. The effects of temperature, the reducing agent beta-mercaptoethanol (BME), and proteinase inhibitors on the level of antigenic activity of recovered HBsAg were determined.
Intake of purple sweet potato beverage affects on serum hepatic biomarker levels of healthy adult men with borderline hepatitis.
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OBJECTIVE
To examine the effect of purple sweet potato (PSP) beverage rich in acylated anthocyanins on serum hepatic biomarkers in healthy Japanese men.
METHODS
A randomized, double-blind, placebo-controlled, parallel study.
METHODS
Kumamoto in Japan.
METHODS
Healthy adult men (30-60 years) with
Double-blind, placebo-controlled pilot trial of anthocyanin-rich purple sweet potato beverage on serum hepatic biomarker levels in healthy Caucasians with borderline hepatitis.
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The objective is to evaluate the efficacy of anthocyanin-rich purple-fleshed sweet potato (PSP) beverage on the serum levels of gamma-glutamyl transferase (GGT), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in healthy Caucasians with borderline hepatitis. Forty healthy
Study of the immunogenicity of hepatitis B surface antigen synthesized in transgenic potato plants with increased biosafety.
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Oral immunogenicity of the hepatitis B surface antigen (HBsAg) synthesized in the tubers of marker-free potato plants has been demonstrated. Experiments were performed in the two groups of outbred NMRI mice. At the beginning of investigations, the mice of experimental group were fed the tubers of
Expression of the hepatitis B surface S and preS2 antigens in tubers of Solanum tuberosum.
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In an attempt to develop an edible vaccine, we transformed a recombinant hepatitis B virus (HBV) gene encoding the middle protein of HBV that contains the surface S and preS2 antigen into potato by Agrobacterium-mediated transformation. The HBV gene was under control of either the CaMV 35S promoter,
Immunogenic properties of chimeric potato virus X particles displaying the hepatitis C virus hypervariable region I peptide R9.
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The immunogenic properties of chimeric potato virus X (PVX) particles engineered to display the synthetic R9 peptide have been evaluated. The R9 peptide is a consensus sequence derived from diverse variants of the hypervariable region 1 from the hepatitis C virus (HCV) envelope protein E2. Two
Potato vaccine for hepatitis.
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Immunogenicity of biologically safe potato tubers synthesizing hepatitis B surface antigen.
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Challenges in creating a vaccine to prevent hepatitis E.
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Recombinant hepatitis E virus capsid protein (HEV CP) assembles orally immunogenic virus-like particles (VLP) when expressed in an insect cell system. We used plant expression cassettes, pHEV101 and pHEV110, for transformation of potato to express HEV CP, and 10 independent transgenic lines of
The antigen of hepatitis delta virus: examination of in vitro RNA-binding specificity.
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The only known protein of hepatitis delta virus (HDV), the delta antigen, is found both within virus particles and within the nucleus of the infected cell, where it has one or more roles essential for RNA genome replication. Others have demonstrated that the antigen has the ability, in vitro, to
Structural characterization of plant-derived hepatitis B surface antigen employed in oral immunization studies.
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Several subunit vaccine antigens have been successfully expressed in plants and recently the hepatitis B surface antigen (HBsAg), expressed in potatoes, was shown to be orally immunogenic in animal studies. However, to date, a detailed analysis of the plant-derived antigen is lacking. Herein, we
Immunoglobulin M-specific serologic testing in an outbreak of foodborne viral hepatitis, type A.
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Ninety-seven symptomatic and five asymptomatic infections with viral hepatitis, type A (102 cases) were identified in members, guests and employees of a private country club in an outbreak associated with consuming food and ice prepared or handled by an employee of the club's kitchen pantry.
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