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Pagina 1 a partire dal 21 risultati
Poly-L-Lysine Inhibits Tumor Angiogenesis and Induces Apoptosis in Ehrlich Ascites Carcinoma and in Sarcoma S-180 Tumor
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Background: This study focuses on the role of Poly-L-lysine (PLL), an essential amino acid, on molecular changes of tumor angiogenesis suppression, pro-apoptotic and anti-apoptotic gene expression after treatment on Ehrlich ascites carcinoma (EAC) and solid sarcoma-180 tumor cells bearing mice.
Effect of poly I:C/poly-L-lysine (poly ICL) on the development of murine osteogenic sarcoma.
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Poly I:C/poly-L-lysine (poly ICL) was effective in preventing or delaying the development of osteogenic sarcoma (OGS) in mice. C57BL/6J mice were inoculated subcutaneously with 5 X 10(4) OGS cells and treatment was evaluated by palpable tumor development and subsequent day of death. A significant
Uptake of L-histidine, L-proline, L-ornithine, L-lysine and L-methionine by brain tissue in vitro: a comparison with uptake by sarcoma RD3 and other tissues.
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Involvement of tumour necrosis factor in monocyte-mediated rapid killing of actinomycin D-pretreated WEHI 164 sarcoma cells.
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Human and murine monocyte-macrophages kill actinomycin D (ActD)-treated WEHI 164 sarcoma cells in a 6-hr 51Cr-release assay (drug-dependent cellular cytotoxicity, DDCC). In this study, we have investigated the cytotoxic activity of human recombinant tumour necrosis factor (hrTNF) against untreated
Photochemical internalization enhances the cytotoxic effect of the protein toxin gelonin and transgene expression in sarcoma cells.
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Further advantages in the treatment of soft-tissue sarcomas will only be achieved by tailoring the adjuvant therapy after surgery. The photochemically directed release of macro-molecules from endosomes and lysosomes into the cytosol is a novel technology, named photochemical internalization (PCI),
Enhancing effect of polyamines on yield of film sarcoma.
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The salts of the natural polyamines, protamine sulphate, clupeine sulphate, spermidine phosphate, putrescine dihydrochloride and spermine diphosphate, and of the synthetic poly-L-lysine hydrobromide (mol. wt 85 k) and poly-L-arginine hydrochloride (mol. wt 50 k) were tested for their effect on film
Systemic antiangiogenic activity of cationic poly-L-lysine dendrimer delays tumor growth.
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This study describes the previously unreported intrinsic capacity of poly-L-lysine (PLL) sixth generation (G(6)) dendrimer molecules to exhibit systemic antiangiogenic activity that could lead to solid tumor growth arrest. The PLL-dendrimer-inhibited tubule formation of SVEC4-10 murine endothelial
Effective suppression of the Kirsten rat sarcoma viral oncogene in pancreatic tumor cells via targeted small interfering RNA delivery using nanoparticles.
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OBJECTIVE
The objective of this study was to establish an efficient carrier for small interfering RNA (siRNA) delivery targeting pancreatic tumor cells.
METHODS
A copolymer consisting of a single-chain variable fragment targeted to human CD44 variant 6 (scFv(CD44v6)) functional group conjugated to
Effects of protease inhibitors on chemical induction of type C virus.
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A role for proteolysis during chemical induction of endogenous xenotropic Type C virus from Kirsten sarcoma virus-transformed mouse cells was examined. Two distinct classes of protease inhibitors, the trypsin inhibitor, alpha-N-tosyl-L-lysine chloromethyl ketone, and two naturally occurring
Preparation and characterization of antibodies to O-phosphotyrosine and their use for identification of phosphotyrosine-containing proteins.
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To facilitate the identification of phosphotyrosine (P-tyr)-containing proteins, rabbit polyclonal antibodies and mouse monoclonal antibody specifically reactive to P-tyr were prepared by hyperimmunizing the animals with P-tyr-conjugated bovine serum albumin or poly-L-lysine. As determined by a
Transcriptional regulation of a contractile gene by mechanical forces applied through integrins in osteoblasts.
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We examined mechanotranscriptional regulation of the contractile gene, alpha-smooth muscle actin (SMA), in osteoblastic cells. Tensile forces were applied through collagen-coated magnetite beads to ROS17/2.8 cells. These cells were desmin-, vimentin+ and expressed low levels of SMA. After force
Comparison of wheat germ agglutinin- and phorbol myristate acetate-mediated triggering for macrophage H2O2 release: susceptibilities to various macrophage inhibitors.
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Murine peritoneal macrophages (M phi S) elicited by a single injection of zymosan A showed a higher responsiveness to the wheat germ agglutinin (WGA)-mediated triggering for H2O2 release than M phi S activated by double injections of the agent. On the contrary, the response to phorbol myristate
Improved function of microencapsulated hepatocytes in a hybrid bioartificial liver support system.
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Conventional methods of microencapsulating isolated hepatocytes with Type I collagen matrix have provided metabolic liver support in experimental animal models of acute liver failure and congenital metabolic liver disease. We compared the biological function of transplanted microencapsulated
Polycation-DNA complexes for gene delivery: a comparison of the biopharmaceutical properties of cationic polypeptides and cationic lipids.
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DNA plasmids formed particulate complexes with a variety of cationic polyamino acids and cationic lipids, which were used to transfect mammalian cells in culture. Complexation was studied by assaying for exclusion of ethidium using a fluorometric assay, which indicated that complexation with
Transgene expression in the brain stem effected by intramuscular injection of polyethylenimine/DNA complexes.
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Gene delivery into the CNS without tissue destruction is challenging. As neurons are capable of taking up exogenous particulates from the muscles that they innervate, we investigated the feasibility of achieving gene transfer in CNS neurons by peripheral intramuscular injection of plasmid DNA
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